采用盆栽试验,设4个施钼水平,即0、0.2、0.4、0.8 mg/kg,研究了施钼对滇重楼生长、养分元素含量和根茎总皂甙含量的影响。结果表明,与不施钼相比,施钼显著提高了滇重楼株高、光合速率、生物量和根茎产量,分别以0.4、0.2、0.4、0.2 mg/kg处理最高。中低施钼水平(0.2、0.4 mg/kg)下,叶和新根茎氮含量呈增加的趋势,高施钼水平(0.8 mg/kg)可显著降低叶和新根茎氮含量;施钼降低了叶和新根茎中的磷含量,但增加了地上茎中的磷含量;施钼增加了叶中钾含量,但降低了茎中钾含量。各施钼处理叶、地上茎、新根茎中钼含量均显著高于不施钼处理;随施钼水平的增加,各部位钼含量呈先增加后降低的趋势。与不施钼相比,各施钼处理新根茎中总皂甙含量显著增加,以Mo 0.8 mg/kg处理最高。综合考虑滇重楼生长、药用部位生物量累积、钼和总皂甙含量,本试验条件下,以施Mo 0.2~0.4 mg/kg较为适宜。
A pot experiment was carried out to study the effects of Mo application on growth,biomass,nutrient contents in different parts and total saponin content in rhizome of Paris polyphylla var.yunnanenis.There were four levels of Mo application: 0,0.2,0.4 and 0.8 mg/kg.The results show that the plant height,photosynthesis rate,biomass and rhizome are increased significantly with the application of Mo,the highest values are 0.4,0.2,0.4 and 0.2 Mo mg/kg respectively.There is an increasing tendency of N contents in leaves and new rhizome under the medium and low levels of Mo(0.2,0.4 mg/kg)compared with no Mo treatment(control),while the N contents in leaves and new rhizome are significantly decreased under the high level of Mo(0.8 mg/kg).The P contents in leaves and new rhizome are decreased under the Mo applications,while the P contents in stems are increased.The K contents in leaves are increased under the Mo applications,while the K contents in stems are decreased.The Mo contents in leaves,stems,and new rhizome of the Mo application treatments are significantly higher than those of the control,and the tendency is increased at first and then decreased.The total saponin contents in new rhizome of the Mo application treatments are significantly increased compared with control,the highest of which is the Mo 0.8 mg/kg treatment.Comprehensively considering the growth status,increased weight and saponin content of rhizome,Mo contents in different parts,the suitable level of Mo application is between 0.2 and 0.4 mg/kg for Paris polyphylla var.yunnanenis in this experiment.