中文摘要：

用低维生素E（VE）和添加不饱和脂肪酸的日粮饲喂雏鸡建立Ve缺乏雏鸡模型，采用比色法和半定量RT—PCR法分别检测脾脏淋巴细胞Caspase3活性及Caspase-3mRNA的表达水平。结果显示，VE缺乏组雏鸡脾脏淋巴细胞Caspase-3活性和Caspase-3mRNA丰度高于对照组，组间差异均极显著（P〈0．01）。结果表明，Caspase-3活性及Caspase-3mRNA丰度的改变，是VE缺乏雏鸡脾脏淋巴细胞凋亡的调控机制之一。

英文摘要：

The activity of Caspase-3 and expression of Caspase-3 mRNA of splenic lymphocytes in the chicken with Vitamin E （VE） deficiency were detected by colorimetry and semi-quantitative reverse transcriptase-polymerase chain reaction （RT-PCR）. The results showed that the activity of Caspase-3 and abundance of Caspase-3 mRNA was higher in VE deficiency chicken than those of control （P〈0.01）. The results indicate that the changes of Caspase-3 activity and Caspase-3 mRNA abundance are one of the regulation mechanisms on lymphocyte apoptosis in the chicken with VE deficiency.