从自然界筛选出98株产还原型谷胱甘肽(GSH)的酵母菌,发酵30 h,分别测定胞内GSH含量,其中2053#菌株的GSH产量最高.初步发酵实验结果表明,2053#酵母菌株在发酵28 h时菌体生物量(细胞干重)达到最大值11.55 g/L,而胞内GSH含量在发酵30 h时达到最大值49.74 mg/L.在常规生理生化方法鉴定的基础上,结合18S rDNA序列分析表明2053#与Candida parapsilosis(近平滑假丝酵母)的相似性达99.75%,为Candida parapsilosis的1个亚种.
98 strains of yeast producing GSH were isolated from nature. Intracellular GSH was extracted by a simple and effective method. The yield of intracellular GSH of 2053# was the highest in them after 30 h of fermentation. The results of fermentation showed that the highest dry cell weight of 2053# reached 11.55 g/L at 28 h, while the highest intracellular GSH concentration arrived 49.74 mg/L at 30 h. In addition to general physiological and biochemical properties, the strain was identified by 18S rDNA sequence and systematic analysis. The results showed that 18S rDNA sequence of the strain had similarity of 99. 75 % with Candida parapsilosis, suggesting that the strain is a subspecies of Candida parapsilosis.