欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
将超声波萃取手段引入到烟叶中3-磷酸甘油脱氢酶提取方法中，并通过正交实验确定了超声波萃取烟叶中3-磷酸甘油脱氢酶（GPDH）的最佳条件为：超声波破碎功率为75w，超声波破碎间歇时间为2s，超声波破碎时间为3s，料液比为1：15g／ml；在此最佳条件下测得烟叶中3-磷酸甘油脱氢酶为0．3937U／mg蛋白，均比其他提取手段的酶活要高。
英文摘要：
Nicotiana tabacum is an important and classical model plant which can respond to the change of environmental conditions by accumulating osmoprotectants, such as glycerol and proline which contribute to the re-establishment of homeostasis when exposed to various adverse environmental stresses, such as drought, salinity, high and low temperatures. The optimization of ultrasonic extraction （UE） conditions of glycerol-3- phosphate dehydrogenase （GPDH） of tobacco leaf have been built by orthogonal test. It showed that optimum of the powers, treatment times, slot times and leaf-to-solvent ratios of UE was 75w, 2h, 2s, and 1 ： 12 g/ml, respectively. Under these conditions, the activity of GPDH has been tested as 0. 3937U/mg protein, which was higher than other extraction methods such as liquid nitrogen and grinding on ice bath. According to investigation, it is the first description of determination of content of GPDH with ultrasonic in tobacco. It could provide basis for the further research in the relation of content of glycerol and osmotic pressure in tobacco.
同期刊论文项目
同项目期刊论文
Cloning and characterization of an NAD+-dependent glycerol 3-phosphate dehydrogenase gene from Candi
Agrobacterium tumefaciens-mediated transformation of industrialized strain Candida glycerinogenes us
Comparative Characterization of Genes Encoding Glycerol 3-phosphate Dehydrogenase From Candida glyce
Cloning and characterization of a NAD(+)-dependent glycerol-3-phosphate dehydrogenase gene from Cand
Transformation of Industrialized Strain Candida glycerinogenes with Resistant Gene zeocin via Agroba
期刊信息
位置：