中文摘要：

禁止的因素(MIF ) 玩的哺乳动物的巨噬细胞移植一个重要角色在鱼对 MIF 相当或相同事物的角色作为在象败血病，而是很少一样的煽动性的疾病的致病的一个不可缺少的调停人被知道败血病。作者用赛跑途径在大黄 croaker Pseudosciaena crocea (LycMIF ) 克隆 MIF 相当或相同事物。LycMIF 的全身的 cDNA 是 634 个底并且包含了编码 115 氨基酸残余的蛋白质的 345 个底的 ORF。是由 RT-PCR 和 QRT-PCR 试金示威了， MIF mRNAs 组成地在 11 选择纸巾被表示并且充满大脑和肝。而且，在肝的 LycMIF 抄本和头肾对细菌感染应答并且能显著地起来调整。我们的结果提供鱼 MIF 在鱼 vibrosis 的致病被含有并且响应细菌感染起一个重要作用的第一条直接证据。

英文摘要：

Mammalian macrophage migration inhibitory factor （MIF） plays an important role as an indispensable mediator in the pathogenesis of inflammatory disease like septicemia, but little is known about the role of MIF homologue in fish septicemia. The authors have cloned the MIF homologue in large yellow croaker Pseudosciaena crocea （LycMIF） using RACE approach. The full-length cDNA of LycMIF was 634 bases and contained an ORF of 345 bases encoding a protein of 115 amino acid residues. As demonstrated by RT-PCR and QRT-PCR assay, MIF mRNAs were constitutively expressed in 11 selected tissues and were abundant in brain and liver. Moreover, the LycMIF transcripts in the liver and head kidney were responsive to bacteria infection and could be significantly up-regulated. Our results provide the first direct evidence that fish MIF was implicated in pathogenesis of fish vibrosis and play an important role in response to bacteria infection.