中文摘要：

从患病大黄鱼病灶中分离出病原哈维氏弧菌,并人工回接感染大黄鱼,病鱼出现典型的皮肤溃疡症。测定大黄鱼血液各项指标发现,病鱼单核细胞、嗜中性粒细胞、血栓细胞百分率明显下降,而淋巴细胞百分率增加,均呈极显著差异（P〈0.01）。患病大黄鱼总蛋白为（12.8±3.95）g/L,对照组鱼为（19.4±4.33）g/L,病鱼球蛋白、甘油三脂、血糖含量也明显低于对照组（P〈0.01）。然而病鱼谷丙转氨酶为（37±5.11）IU/L,对照组为（28±5.54）IU/L;病鱼谷草转氨酶为（164±47.19）IU/L,对照组为（117±21.84）IU/L,均呈极显著差异（P〈0.01）。研究结果说明,病鱼肝脏和肾脏组织受损。对患病大黄鱼进行病理组织学观察,结果发现病鱼病变组织头肾、肝出现不同程度的变性以及坏死。细胞超微病变观察发现,头肾肾小管上皮细胞变性、坏死。肝细胞胞浆内脂滴积累多。血清指标的测定可以作为大黄鱼哈维氏弧菌病细菌感染的指标。

英文摘要：

The pathogen Virio harveyi isolated from the focus of diseased Pseudosciaena crocea was infected back to the healthy P.crocea artificially.The challenged fish indicated the typical ulcered syndrome.The blood chemistry parameters were tested.The challenged fish indicated significantly（P0.01） lower percentages of monocytes,polymorphonuclear neutrophil granulocytes（PMN） and thrombocytes but higher percentages of leucocytes;it differed from the control fish.Significant decreases in total protein,globulin,triacyl glycerol and blood glucoe concentrations were found in the challenged fish compared to the control fish（P0.01）,with the total protein values at（12.8±3.95） g/L and（19.4±4.33） g/L,respectively.Significant increases of alanine aminotransferase（ALT） and aspartate aminotransferase（AST） concentrations were found in the challenged fish compared to the healthy fish（P0.01）.The concentrations of ALT and AST were（37±5.11） IU/L and（164±47.19） IU/L in the challenged fish,while in the control fish they were（28±5.54） IU/L and（117±21.84） IU/L.The results indicated liver and kidney damage,and disturbances in hepatic and osmoregulatory function of P.crocea.Observations by histopathology showed that head kidney and liver of the tested P.crocea were severely damaged and various denaturalization and necrosis.Denaturalization and necrosis emerged in the epithelial cell of renal tubule under electronic microscope.Fat was accumulated in the cytoplasm of hepatic cells.The serum chemistry parameter determination was a good indicator of bacterial infection of the challenged fish.