中文摘要：

目的探讨职业慢性心理应激与血清热休克蛋白70（HSP70）和肿瘤坏死因子-α（TNF-α）的关系。方法采用病例一对照研究设计，于2011年10月-2012年10月，选取宁夏回族自治区2家医院中确诊的20～60岁无血缘关系的代谢综合征（MS）患者作为病例组，共计622例。同期选取前往上述2家医院进行健康体检的健康人群作为对照组，共600名。通过问卷方式调查研究对象性别、年龄、民族、身高、体重、吸烟、饮酒、运动等情况。经知情同意，均采集空腹静脉血样10ml进行实验室生化指标检测。采用ELISA测定血清中HSP70与TNF-α表达量。采用经修订的职业紧张量表（OSI），调查研究对象职业慢性心理应激因素及应激水平。采用偏相关分析法分析职业慢性心理应激总分与HSP70、TNF-α相关性；以HSP70、TNF-α为自变量，以职业慢性心理应激总分为因变量，构建多元线性回归分析方程，采用方程决定系数R。判断方程的拟合程度。结果全部调查对象慢性心理应激因素总得分为（136．65±16．19）分。其中，轻度应激水平组313名、中度588名、重度321名，慢性心理应激因素得分分别为（119．96±13．30）、（135．33±3．23）、（155．33±13．55）分。病例组调查对象HSP70在轻度、中度及重度职业慢性心理应激水平组表达水平分别为（29．88±30．08）、（36．38±30．08）、（27．16±23．77）ng／ml（F=6．85，P=0．001）；对照组分别为（27．64±9．89）、（39．78±29．77）、（3．94±3．09）ng／ml（仁125．71，P〈0．001）。多元线性回归分析结果显示，心理应激水平与HSP70为负线性关系，与TNF-α为正线性关系，拟合回归方程为y=-0．07x1＋0．011x2＋136．88。偏相关分析结果显示，职业慢性心理应激总分与HSP70呈负相关（r=-0．11，P〈0．001），与TNF．仅呈正相关（r=0．11，P〈0．001）。全体调查对象中HS

英文摘要：

Objective To investigate the relationship between occupational chronic psychological stress with heat shock protein 70 （HSP70） and tumor necrosis factor-alpha （TNF-α）. Methods Using case-control study design, we selected 622 cases in 20 to 60 years old and unrelated patients with metabolic syndrome as the case group between October 2011 and October 2012 at two hospitals of Ningxia hui autonomous region. At the same time, we selected 600 healthy people from health check-up crowd in the above two hospitals as control group. The the research objects were sex, age, nation, height, weight, smoking, drinking, exercise, and so on. After informed consent, all the research objects were collected fasting venous blood samples 10 ml in order to proceed laboratory testing of biochemical indicators. The expression of HSPT0 and TNF-α in serum was determined by ELISA. Using the revised occupational stress inventory （OSI） to survey the occupational chronic psychological stress factors and stress level of research object. The correlation of occupational chronic psychological stress scores with HSP70 and TNF-α was investigated by partial correlation analysis. We built a multivariate linear regression equation With HSP70 and TNF alpha as the independent variable and occupational chronic psychological stress scores as the dependent variable, using equation of the determination coefficient R2 to judge the degree of fitting equation. Results The total points of chronic stress factors in all respondents was （136.65±16.19）. Among them, the mild stress level group was 313, moderate was 588, severe was 321, chronic heart stress factors scores were （119.96±13.30）, （135.33±3.23）, （155.33± 13.55） points, respectively. In the case group subjects, the expression of HSP70 in mild, moderate and severe occupational chronic psychological stress levels were （29.88± 30.08）, （36.38± 30.08）, （27.16±23.77） ng/ml （F=6.85, P=0.001）. The control group were （27.64±9.89）, （39.78±29.77）, （3.94