氯代有机溶剂与酚类化合物复合污染地下水的现象较为普遍,但地下水环境中溶解氧缺乏,好氧微生物降解作用难以发挥。本文驯化好氧微生物Burkholderia cepacia耐受高浓度H2O2,并利用H2O2作为供氧源降解地下水环境中的三氯乙烯(TCE)和苯酚。结果表明,对于Burkholderia cepacia的生长过程,H2O2的最佳投加量为4mmol/L,加入H2O2后,会短暂抑制TCE降解,但最终大幅提高了TCE的降解率,达到79.80%。动力学分析表明,在H2O2供氧条件下,共代谢苯酚与TCE的比降解速率可以由Monod模型和Haldane模型分别进行描述,且显著相关。研究结果为好氧微生物控制地下水环境中的TCE和苯酚复合污染提供了科学依据。
Combined pollution of chlorinated solvent and phenolic compounds is very common in the groundwater.However,the absence of dissolved oxygen(DO)in the groundwater renders aerobic degradation difficult.Burkholderia cepacia was acclimated to tolerate high concentration of H2O2 and degrade trichloroethylene(TCE)and phenol using H2O2 as an oxygen source in the groundwater.The results showed that the optimal concentration of H2O2 was 4 mmol/L for the growth of Burkholderia cepacia.After adding H2O2,active biodegradation of TCE followed after a short lag phase,but it significantly improved the biodegradation rate of TCE.And the efficiency of TCE degradation reached up to 79.80%.The degradation curves of phenol and TCE were represented by the Monod and Haldane model respectively in the presence of H2O2,and there is a well relevance between each other.This study findings provided a scientific evidence for the aerobic degradation of TCE-phenol co-contaminated groundwater.