中文摘要：

【目的】建立螺旋藻藻种的超低温保存法,并探究该方法对不同种类螺旋藻藻种保存的适用性。【方法】采用碘量法筛选出耐低温螺旋藻藻株,通过单因素和正交试验设计对耐低温螺旋藻超低温保存法进行条件优化,并以优化后的超低温保存法对8株不同种类的螺旋藻进行保藏实验。【结果】FACHB-351为筛选出的耐低温螺旋藻藻株;优化后的超低温保存方案为：以10%蔗糖溶液做冷冻保护剂,将藻丝体密度为1.0×107 CFU/m L的藻悬液于4°C驯化72 h,再将藻液和保护剂分别在0°C预冷30 min后混匀,混匀后于0°C停留3 h,然后投入液氮保存。保藏实验结果表明,保藏6个月时除了耐低温性较差的FACHB-350、FACHB-1070、FACHB-902螺旋藻存活率为0,不能恢复生长繁殖,其它5种耐低温性较好的螺旋藻均能在一定时间内恢复正常的生长繁殖,其中FACHB-351的存活率最高,为39.33%。【结论】建立的超低温冷冻保存法可用于耐低温性较好的螺旋藻藻种的长期保存。

英文摘要：

[Objective] This study aimed to establish the cryopreservation technique of Spirulina that with low temperature tolerance and research the preservative applicability of this method to the different Spirulina. [Methods] We screened out the low temperature tolerant Spirulina by iodometric method. This paper used the single factor analysis and orthogonal experimental designed method to optimize the conditions to the cryopreservation technique of Spirulina with low temperature tolerance, and then used this optimized method to preserve the eight different kinds of Spirulina. [Results] The result showed that the FACHB-351 Spirulina was the best one to endure the low temperature, and the optimal cryopreservation conditions were obtained as follows： using the 10% sucrose solution as the cryoprotectant, putting the algal cell density of 1.0×107 CFU/m L in the 4 °C refrigerator liquid and cultivating for 72 h, mixing up the algae fluid and protective agent after precooling at 0 °C for 30 minutes respectively, and then preserving it in the liquid nitrogen after the mixture liquid stayed at 0 °C for 3 h. This experimental result indicated that FACHB-350, FACHB-1070, FACHB-902 were no-resistant to low temperature and lost cell activity with the survival rate of 0% after preservation for six months, the rest five kinds of Spirulina could resurrect during a certain period of time for the growth of normal. And the FACHB-351 showed be the highest survival rate 39.33%. [Conclusion] This study established the cryopreservation method which was suitable for the preservation of good low temperature resistance Spirulina.