中文摘要：

目的应用甲状腺球蛋白（Tg）诱导大鼠建立实验性自身免疫性甲状腺炎（EAT）动物模型，观察不同碘营养状态下大鼠甲状腺的病理改变和CD4＋T淋巴细胞的浸润程度。方法4周龄雌性Lewis大鼠135只（体质量约80g），按体质量采用随机数字表法分为对照组（NC）、模型组（TG）、高碘Ⅰ组（HⅠ）、高碘模型Ⅰ组（HⅠ＋TG）、高碘Ⅱ组（HⅡ）和高碘模型Ⅱ组（HⅡ＋TG），NC、HⅠ、HⅡ组每组20只大鼠，TG、HⅠ＋TG、HⅡ＋TG组每组25只大鼠。HⅠ、HⅠ＋TG组大鼠饮用含碘离子为25．7mg／L的去离子水，HⅡ组和HⅡ＋TG组大鼠饮用含碘离子为423．3mg／L的去离子水，NC和TG组饮用蒸馏水。TG、HⅠ＋TG、HⅠI＋TG组大鼠皮下注射含8．0g／L他的不完全弗氏佐剂0．1mI，两周1次，共3次。各组大鼠于建立模型后第8周，收集尿液；于建立模型后第15周末处死动物，采集血样、取甲状腺组织。砷铈催化分光光度法测定大鼠尿碘；苏木精．伊红（HE）染色法观察甲状腺组织病理学改变；免疫组织化学方法检测甲状腺组织炎性细胞水平。结果各组大鼠甲状腺相对质量组间比较差异有统计学意义（F=44．428，P〈0．05）。其中与NC组（0．08525mg／g）比较，各实验组大鼠甲状腺相对质量（TG、HⅠ、HⅠ＋TG、HⅡ、HⅡ＋TG组：0．09522、0．09285、0．09748、0．09655、0．09533mg／g）均升高（P均〈0．05）；各组大鼠尿碘组间比较差异有统计学意义（χ2=106．4，P〈0．05）。其中TG、HⅠ、HⅠ＋TG、HⅡ、HⅡ＋TG组大鼠尿碘（800．08、18633．20、13869．00、87889．97、61661．51μg／L）均高于NC组（456．45μg/L、P均〈0．05）。各组大鼠甲状腺病理改变随着碘摄入的增加逐渐加重。NC组大鼠甲状腺结构正常；TG、HⅠ组甲状腺滤泡间少量淋巴细胞浸润；HⅠ＋TG组可见淋巴细胞浸润到滤泡内部；HⅡ、HⅡ＋TG组滤泡

英文摘要：

Objective To establish a experimental autoimmune thyroiditis（EAT） rat model and to observe pathological change levels of CD4 ＋ T lymphocyte infiltration in thyroid tissues under different iodine nutrient conditions. Methods One hundred and thirty-five four weeks old female Lewis rats（body weight about 80 g） were divided into control （NC）, model （TG）, high iodine- Ⅰ （HⅠ ）, high iodine and model- Ⅰ（HⅠ ＋ TG）, high iodine-Ⅱ （HⅡ ）, high iodine and model-Ⅱ （HⅡ ＋ TG） groups according to body weight by random number table. There were 20 rats in NC, HⅠ and HⅡ groups, and 25 rats in TG, HⅠ ＋ TG and H Ⅱ ＋ TG groups. The rats of HⅠ and HⅠ ＋ TG groups drank deionized water containing iodine 25.7 mg/L; rats of HⅡ and HⅡ ＋ TG groups drank deionized water containing iodine 423.3 mg/L; rats of NC and TG groups drank distilled water. Rats of TG, HⅠ ＋ TG and HⅡ ＋ TG groups were immunized with 0.1 ml thyroglobulin（Tg, 8.0 g/L） in incomplete Freunds adjuvant（IFA）, once two weeks for three times. Urine samples were collected after immunization for 8 weeks; after immunization for 15 weeks, rats were killed, blood samples and thyroid tissues were taken. Urinary iodine was determined by arsenic-cerium catalytic spectrophotometry; pathological changes in thyroid tissue were observed by Hematoxylin-Eosin （HE） method ; immunohistochemical method was used to identify the infiltration of inflammatory cells in thyroid tissue. Results The difference of rat thyroid relative quality between groups was statistically significant （F = 44.428, P 〈 0.05）. Compared with NC group（0.085 25 mg/g）, thyroid relative qualities were increased in experimental groups （TG, HⅠ , HⅠ ＋ TG, H H, HⅡ ＋ TG groups： 0.095 22, 0.092 85, 0.097 48, 0.096 55, 0.095 33 mg/g, all P 〈 0.05）. The difference of urinary iodine between groups was statistically significant（χ2 = 106.4, P 〈 0.05）. Compared with NC group（456.45 μg/L）, urinary iodine