欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
Simultaneous and sequential adsorptions of bovine serum albumin(BSA) and bovine hemoglobin(bHb) was investigated on anion ion-exchange media Q Sepharose FF in batch adsorption experiments to elucidate the mechanism of multi-component protein adsorption.BSA is always preferential adsorbed with bHb inhibited in binary adsorption.Influence on binary adsorption of factors such as pH,salt concentration,initial concentration ratios of proteins and the adsorption sequence are determined.Three models have been tested and compared to describe the competitive binary adsorption of BSA and bHb in all conditions studied.Among the extended Langmuir model gives the best predict of adsorption of the stronger adsorbed protein when its adsorption is not very strong in simultaneous adsorption,the Statistical thermodynamic(ST) model gives the best description of weaker adsorbed protein in simultaneous adsorption,the Steric mass-action(SMA) model is most appropriate multi-component model considering all different conditions for both proteins.
英文摘要：
Simultaneous and sequential adsorptions of bovine serum albumin(BSA) and bovine hemoglobin(bHb) was investigated on anion ion-exchange media Q Sepharose FF in batch adsorption experiments to elucidate the mechanism of multi-component protein adsorption.BSA is always preferential adsorbed with bHb inhibited in binary adsorption.Influence on binary adsorption of factors such as pH,salt concentration,initial concentration ratios of proteins and the adsorption sequence are determined.Three models have been tested and compared to describe the competitive binary adsorption of BSA and bHb in all conditions studied.Among the extended Langmuir model gives the best predict of adsorption of the stronger adsorbed protein when its adsorption is not very strong in simultaneous adsorption,the Statistical thermodynamic(ST) model gives the best description of weaker adsorbed protein in simultaneous adsorption,the Steric mass-action(SMA) model is most appropriate multi-component model considering all different conditions for both proteins.
同期刊论文项目
同项目期刊论文
Dynamic behavior of binary component ion-exchange displacement chromatography of proteins visualized
Molecular dynamics simulation and experimental validation for the effect of pH on protein desorption
Development of a displacer-immobilized ligand docking scheme for displacer screening for protein dis
Molecular mechanism of the effects of salt and pH on the affinity between protein A and human immuno
Effect of dextran layer on protein uptake to dextran-grafted adsorbents for ion-exchange and mixed-m
Molecular dynamics simulation of the effect of ligand homogeneity on protein behavior in hydrophobic
Mono-sized microspheres modified with poly(ethylenimine) facilitate the refolding of like-charged ly
Molecular basis of the initial platelet adhesion in arterial thrombosis: Molecular dynamics simulati
Trace adsorption of positively charged proteins onto Sepharose FF and Sepharose FF-based anion excha
期刊信息
位置：