中文摘要：

目的：利用脂质体转染肿瘤坏死因子受体2（TNFR2）于喉鳞癌裸鼠模型中，探讨转染的最佳剂量及时间。方法：构建喉鳞癌裸鼠模型，将不同剂量TNFR2于不同时间转染至模型中，利用流式细胞术及免疫组织化学法测定TNFR2的表达。结果：鳞状细胞癌荷瘤裸鼠的瘤组织基本不表达TNFR2。转染TNFR2后，48h蛋白表达达高峰，主要为细胞膜表达。给瘤体内转染5μg TNFR2效果最佳，蛋白表达水平最高。结论：TNFR2的最佳转染剂量为5μg，48h可达高峰。此结果为喉鳞癌的基因治疗提供了依据。

英文摘要：

Objective：To establish the best dose and time of the transfection of tumor necrosis factor receptors 2 （TNFR2） in vivo. Methods： Flow eytometry and Immunohistochemistry were needed to measure the TNFR2 expression. Results： Results showed that 5 μg TNFR2 was the best dose and 48 h was the better transfection time. TNFR2 was not expressed in the empty vetor group. The membrane expression,which was positive,was at its peak 48 h after transfection Of TNFR2 and the transfection of 5μg TNFR2 showed positive results and the protein expression was at its highest peak. Conclusion：The best transfection dose of TNFR2 is 5 μg and can reach its peak in 48 h so it provides basic proof for the genetic treatment of the laryngeal squamous cacinoma.