中文摘要：

2009-2010年以陆地棉标准系TM-1和海岛棉标准系3-79作为对照,将陆地棉的7个半野生种系64份种质资源进行了简单重复序列（SSR）分子标记的遗传多样性和亲缘关系研究。112对SSR引物共检测出502个等位位点,其中多态性位点392个,约占总位点数的78%。每对引物扩增出2~10个等位位点,平均约为4.5个,其中多态位点数1~9个,平均约3.5个。供试半野生种系间的遗传相似系数为0.533~0.995,平均0.690,说明陆地棉半野生种系之间存在着丰富的遗传多样性。7个陆地棉半野生种系遗传多样性顺序为：玛利加郎特棉〉尤卡坦棉〉阔叶棉〉帕默尔棉〉莫利尔棉〉雷奇蒙地棉〉尖斑棉。UPGMA聚类结果表明,7个陆地棉半野生种系在相似系数为0.690处被聚为四大类,帕默尔棉和阔叶棉与陆地棉的亲缘关系最近。

英文摘要：

To evaluate the genetic diversity and relationship among the semi-cultivars of 7 G.hirsutum L.races,64 accessions were analyzed with SSR markers,using TM-1（the genetic standard line of G.hirsutum） and 3-79（the genetic standard line of G.barbadense） as checks.A total of 502 gene alleles with 78% polymorphism were detected from 112 SSR primers.The number of DNA bands per primer ranged from 2 to 10 with an average of 4.5,and the polymorphic ones ranged from 1 to 9 with an average of 3.5.The average coefficient of genetic similarity among the tested germplasms was 0.690,ranging from 0.533~0.995,which indicated that the genetic diversity among the semi-cultivars of G.hirsutum L.races were rich.The average coefficient of each G.hirsutum L.race ranged from 0.653~0.796,with the highest of Marie-Galante and the lowest of punctatum,which indicated that the genetic diversity among Marie-Galante is much higher than the other races.Generally,the genetic diversity among the seven races of G.hirsutum L.is in order as： Marie-GalanteyucatanenselatifoliumpalmeriiMorrillirichmondiipunctatum.The UPGMA cluster analysis showed that the races of G.hirsutum could be clustered into four groups when genetic similarity coefficient was given as 0.690,and palmerii as well as latifolium were more closed to the cultivated upland cotton than others.