中文摘要：

目的评价阿仑膦酸钠对骨质疏松大鼠关节软骨的保护作用。方法选取36只6月龄的SD雌鼠随机分为3组（每组12只）：假手术组（SHAM）仅手术，不切除卵巢；去卵巢＋生理盐水（OVX＋NS）组和OVX＋阿仑膦酸钠（ALEN）组切除双侧卵巢，其中OVX＋ALEN组给予阿仑膦酸钠液（3mg／ml）每周36mg/kg剂量灌胃，SHAM组和OVX＋NS组给予同等剂量0．9％生理盐水灌胃，于灌胃后4、8、12周时取大鼠近端胫骨常规脱钙，苏木索-伊红（HE）染色切片测量软骨厚度，甲苯胺蓝和Ⅱ型胶原免疫组织化学染色，切片用图像分析仪测量平均灰度值。结果4周时各组的软骨厚度相近：8、12周时OVX＋NS组与OVX＋ALEN组及SHAM组相比软骨厚度变薄（P〈0．05）；12周时OVX＋NS组Ⅱ型胶原免疫组织化学染色灰度值低于OVX＋ALEN组和SHAM组（P〈0．05）。结论骨质疏松情况下关节软骨的结构、代谢会发生改变，服用阿仑膦酸钠对关节软骨具有保护作用。

英文摘要：

Objective To evaluate the effect of alendronate on articular cartilage of rat knee joint with osteoporosis. Methods Thirty-six 6-month-old female SD （Sprague-Dawley） rats were divided randomly into three groups. Rats in group （OVX＋NS） and group （OVX＋ALEN） were subjected to bilateral oophorectomy, while rats in Sham group subjected to sham-operation. Group （OVX＋ALEN） received a weekly intragastric administration of alendronate solution （36 mg per kg of body weight） while the Sham group and group （OVX＋NS） received normal saline at the same dose. After 4, 8 and 12 weeks, four rats in every group are sacrificed and tibial articular cartilage from these rats were harvested and stained with HE, toluidine blue and type Ⅱ collagen immunohistochemistry. Gray scale of type Ⅱ collagen immunohistochemistry sections were measured by image analyser and the thickness of articular cartilage were measured with HE sections. Results The thickness of articular cartilage of group （OVX＋NS） was lower than group （OVX＋ ALEN） at weeks 8 and 12 （P〈0.05）; Gray scale of type H collagen immunohistochemistry of group （OVX＋ALEN） group was higher than that in group （OVX＋NS） at week 12（P〈0.05）. Conclusion The structure and metabolism of articular cartilage appear altered in osteoporosis, and use of alendronate sodium may be protective for articular cartilage.