中文摘要：

用酵母双杂交系统探索PICK1分子中BAR结构域与PDZ结构域的相互作用．分别构建了pGAD—PDZ和pGBK—BAR重组子，共转入酵母细胞后，涂布于SD（Leu^-，Trp^-．His^-）固体平板培养基，经滤纸复印和显色反应，在8h内观察到只有pGAD—PDZ／pGBK—BAR的表达产物呈阳性，而其它对照pGADT7／pGBKT7、pGAD—PDZ／pGBKT7和pGADT7／pGBK—BAR均呈阴性反应，说明BAR与PDZ结构域具有相互作用．

英文摘要：

The interaction between BAR domain and PDZ domain in PICK1 were studied by using yeast two-hybrid system. Two recombinant plasmids, pGAD-pdz and pGBK-bar were constructed and in turn, co-introduced into yeast AH109. Subsequently, SD （Leu^-, Trp^-,His^-）solid plate medium was used to grow and screen transformed yeast. The colony were copied with whatman No. 5 filter paper and coloured with Z-buffer/X-gal. The result showed that the expresed product of the recombine yeast containing pGAD-PDZ/pGBK-BAR rerealed the position reaction as compared other controls pGADT7/pGBKT7, pGAD-PDZ/pGBKT7 and pGADT7/pGBK-BAR. It suggested that the interaction between BAR domain and PDZ domain was occurred.