中文摘要：

通过RT-PCR的方法获得毛果杨（Populus trichocarpa）PtrWRKY25（Potri.007G078200.1）。经氨基酸比对分析,毛果杨PtrWRKY25蛋白与拟南芥（Arabidopsis thaliana）AtWRKY12、葡萄（Vitis vinifera）VvWRKY2以及苜蓿（Medicago truncatula）MtSTP的WRKY结构域有较高同源性。利用荧光定量及半定量检测PtrWRKY25组织表达量发现该基因在茎中表达量最高,其次为韧皮部和木质部。植物组织切片间苯三酚染色发现PtrWRKY25转基因株系次生壁较野生型也有减少的趋势。检测农杆菌介导在毛白杨中超量表达的PtrWRKY25转基因株系木质素含量发现,相较于与野生型,转基因植株中木质素含量减少约1倍,表明PtrWRKY25可能主要在木质素合成途径中发挥作用。

英文摘要：

A complete fragment representing the PtrWRKY25 （ Potri. 007 G078200. 1 ） isolated from Populus trichocarpa by reverse transcription PCR （ RT-PCR ） displayed significant similarity with other WRKY factors in Arabidopsis＇s At-WRKY12, Vitis vinifera＇s VvWRKY2 and Medicago truncatula＇s MtSTP gene. The expression profiles of PtrWRKY25 showed the highest transcript level in stem and relatively low level in phloem and xylem. Plant tissue sections between phloroglucinol staining found PtrWRKY25 transgenic lines compared with wild-type secondary wall also showed a decrea-sing trend. To investigate its in vivo function, PtrWRKY25 was expressed under control of the CaMV35S promoter in Popu-lus trichocarpa by Agrobacterium-mediated transformation. About 1 time lower accumulation of lignin was detected in transgenic plants harboring PtrWRKY25, which was in agreement with the toluidine blue staining of stem. Our findings suggested that PtrWRKY25 might play more important roles in biosynthesis of lignin.