中文摘要：

分子学方法在研究AM真菌多样性中有着重要作用。为了寻找研究蒙古沙冬青根围土壤AM真菌群落的合适引物,选取3对常用引物进行试验,最终选取2对不同引物AM1/NS31和AMV4.5NF/AMDGR,利用DGGE技术研究内蒙古阿拉善和乌拉特后旗2个样地蒙古沙冬青根围土壤AM真菌多样性及其与土壤因子的相关性,阐明极端干旱环境下AM真菌空间分布及土壤因子的生态作用。结果表明：（1）AM1/NS31引物能够更有效扩增样本AM真菌,表现出丰富的DGGE条带数目。（2）2个样地AM真菌群落结构在种类组成上有较高相似性,不同样地和土层AM真菌群落差异性主要体现在丰富度和优势度上。同一样地,0～20cm土层AM真菌丰富度高于20～40cm土层;同一土层,阿拉善样地AM真菌丰富度更高。不同样本AM真菌优势度有较大差异,Rhizophagus intraradices在所有样本中均有较高优势度。（3）土壤速效P、速效N、速效K和有机质对于AM真菌分布有显著影响。

英文摘要：

To elucidate the spatial distribution of AM fungi and ecological function of soil factors,we sampled the rhizosphere soil of Ammopiptanthus mongolicus from Alashan and Wulate of Inner Mongolia,China and three pairs of AM fungi primer were used.The study showed that：（1）AM1/NS31 primer could amplify AM fungi more efficiently,and the number of DGGE bands was high.（2）The species composition of AM fungal communities was highly similar in two sites,but the species richness and dominance were different between soil depth and sites.AM fungal richness was higher in 0-20 cm soil layer than that in 20-40 cm,and in Alashan site than that in Wulate.Rhizophagus intraradices was dominant in all samples.（3）Soil factors significantly influenced the distribution of AM fungi.