中文摘要：

将酪胺应用于酶联免疫分析,建立了一种新的高灵敏伏安型免疫传感器。利用纳米金的静电吸咐和己二硫醇、巯基乙胺的自组装,将羊抗人IgG抗体固定到金电极表面上,以辣根过氧化物酶标记羊抗人IgG抗体为酶标抗体,以生物素化酪胺为酶底物,利用催化酪胺沉积反应,在传感界面沉积大量生物素,使原始信号得到几何级数的放大。结果表明,通过生物素化酪胺催化放大后,制得的免疫传感器对H2O2的催化能力增大近20倍,检测hIgG在1.5μg/L～22 mg/L范围内有良好的线性关系,检出限为0.1μg/L。用于实际试样的回收率的测定,结果良好。

英文摘要：

A novel and highly sensitive voltammetric enzyme-linked immunosensor was developed based on tyramine oxidation deposition.It was shown that gold nano-particles（colloid Au） could be used as a platform to immobilize antibodies by adsorption.By a sandwich immunossary format with goat-anti-human IgG labled Horseradish peroxidase（HRP） as the second antibody and catalytic amplification by biotin-tyramine,the immunosensor′s catalytic ability to hydrogen peroxide increased nearly 20 times,the sensor exhibitd a linear response to human IgG in the concentration range from 1.5 μg/L-22 mg/L,and the detection limit was 0.1 μg/L,the regression equation could be expressed as Δip（μA） =2.8859c（mg/L）＋17.152 with a correlation coefficient of 0.9872.The immunosensor can be used to quantitatively determine hIgG in the sample of human serum.