中文摘要：

用镀铂碳糊电极电聚酪胺和过氧化聚毗咯固定葡萄糖氧化酶（GOD）制成电流型葡萄糖传感器。铂黑沉积层的微孔结构使电极具有较大的有效表面积，增加了固定的酶量，电极灵敏度提高。通过聚合酪胺（PTY）和过氧化聚吡咯（OPPY），可提高酶电极的稳定性和抗干扰能力。该电极具有响应时间短（〈10s）、响应范围宽（0.01—18mmol／L）、灵敏度高（检出限为5μmol／L）、稳定性好等优点，电极经过60d、约60次反复测试后，响应电流下降至最初值的70％，并具有抗果糖、尿酸和抗坏血酸干扰的特点。

英文摘要：

An amperometric glucose biosensor has been developed based on immobilization of glucose oxidase （GOD） in electrochemically polymerized polytyramine（PFY） and overoxidised polypyrrole （OPPY） on platinized carbon paste electrode （PCPE）. More amount of GOD were fixed on the surface and higher sensitivety was achived by providing a large apparent surface area with the micropores of the electrodeposited platinum particles on the PCPE. Due to the polymerized polytyramine fihn and overoxidised polypyrrole fihn on the PCPE, the sensor was more anti-interferential and more stable. The sensor responds to β-D-glucose linearly over a concentration range of 1 × 10^-5 ~ 1.8 × 10^-2 mol/L, and the detection limit is 5 × 10^ -6 mol/L. The response time of the sensor is less than 10 s and the stability is over 2 months. Due to the permselective characteristics of PTY fihn and OPPY film, the effects of electroactive interferents such as ascorbic acid, uric acid, and fructose can be significantly reduced.