中文摘要：

采用Hiseq2500高通量测序平台完成了铜胁迫和对照条件下哈茨木霉Th-33的转录组测序,共检测出差异表达基因891条,其中上调表达基因570条,下调表达基因321条。GO富集分析显示,共有438个差异表达基因参与到673个不同的功能亚类中;KEGG代谢途径分析显示,共有254个差异表达基因归到167个代谢通路中,其中涉及基因最多的是对氨基苯甲酸甲酯降解和氯烷烃、烯烷烃降解通路。通过对差异表达基因和代谢通路分析,推导出菌株Th-33细胞内可能的铜胁迫应答途径。铜胁迫下,Cu^2＋还原为Cu^＋、铜转运蛋白（copper transporter,Ctr）的转运途径受到抑制,铜离子可能一部分通过吞饮的作用进入哈茨木霉Th-33细胞,可能通过与谷胱甘肽（GST）结合,或通过P-type ATP酶的外排作用将铜离子向质膜外运输以减少对细胞的毒害作用。

英文摘要：

The transcripts of Trichoderma harzianum Th-33 under copper stress and normal conditions were sequenced on a Hiseq2500 instrument. A total of 891 differentially expressed genes （DEGs） were found between the two transcripts, of which 570 genes were up-regulated and 321 genes were down-regulated in expression. GO analysis suggested that total of 438 differentially expressed genes were categorized into 673 functional groups. KEGG analysis revealed that total of 254 genes were annotated in 167 individual pathways and these unigenes were significantly enriched in various known aminobenzoate degradation and chloroalkane degradation. According to the differentially expressed genes and metabolic pathways of T. harzianum Th-33 under copper stress, the copper transport and metabolism pattern in T. harzianum Th-33 were different from that of yeast and T. reesei. Pathways of reducing of copper ions and Ctr transporting were inhibited by copper stress, and part of copper may enter T. harzianum Th-33 cells by endocytosis. It was speculated that copper transferred in T. harzianum Th-33 by combining with glutathione, or transported to the plasma membrane by P-type ATPase.