中文摘要：

目的：研究氧化还原药物对三叉神经节细胞离子通道的调节作用。方法：采用全细胞膜片钳电生理方法，记录氧化还原药物对三叉神经节细胞大电导钙激活钾通道（BKca）的影响。结果：蛋氨酸特异性氧化剂chloramine-T（Ch—T）1mmol／L可轻微增加通道电流幅值，但该作用不能被半胱氨酸还原剂1，4-dithio-DL-threitol（DTT）所逆转。相反，半胱氨酸特异性氧化剂5，5’-dithio-bis（2-nitribenzoic acid）（DTNB）500μmol／L降低BKCa的电流幅值，此作用可被DTT 2mmol／L所逆转。结论：ROS通过氧化调节BKCa通道而参与三叉神经节细胞的功能调节，BKCa通道在氧化应激相关性生理、病理状态下起重要的调节作用。

英文摘要：

To observe redox modulation of ion channel in trigeminal ganglion neurons by oxidants and reducing agents. Methods： The effects of oxidants and reducing agents on maxi-conductance calcium-activated potassium channel in cultured rat trigeminal ganglion neurons by using whole-cell patch-clamp technique. Results： Methionine-specific oxidant chloramine-T （Ch-T） 1 mmol/L slightly increased the current amplitude and this enhancement did not antagonized by DTT. In contrast, cysteine-specific reagent 5, 5＇-dithiobis（2-nitrobenzoic acid） （DTNB） 500μmol/L significantly decreased current amplitude of BKCa channels. The effect was reversed by the reducing agent 2 mmol/L 1, 4-dithio-DL-threitol （DTT）. Conclusion： Reactive oxygen species were definitely involved in regulation of native neuronal function via redox modulation of BKca channels, which are suggested to play compensatory roles under oxidative stress-related conditions.