中文摘要：

转化酶是植物蔗糖代谢与抗逆性形成的关键酶。基于小桐子基因组数据,鉴定到16个小桐子转化酶基因,并对其系统进化、基因结构、理化性质、保守基序及表达特性进行了分析。结果表明,小桐子转化酶基因家族聚类为3个亚家族,中性/碱性转化酶α亚组与酸性转化酶基因包含6或7个外显子,β亚组包含4或5个外显子。同时,酸性转化酶N端都包含信号肽序列,且都鉴定到GH32家族保守的-NDPNG/A-与-MWECV/P-基序。qRT-PCR表达分析显示,JcC-INVD基因在小桐子子叶中表达量最高,而在根中表达量较低,且在叶中属于低温诱导基因。

英文摘要：

Plant invertase is one of critical enzymes in sucrose metabolism and stress resistance formation. The 16 invertase genes wereidentified based on complete genome data of Jatropha curcas,then the evolutionary relationship,gene structure,physical and chemicalcharacteristics,conserved motif,and expression feature were analyzed. The results showed that invertase gene family in J. curcas was clusteredto three subfamilies according to phylogenetic analysis. The α subgroup of neutral/alkaline invertases and acidic invertase gene owned 6-7exons,and β subgroup owned 4-5 exons in gene structure. Signal peptides in N-terminal and conserved motifs of-NDPNG/A-and-MWECV/Pbelonging to GH32 family were identified in acidic invertase protein sequences. q RT-PCR analysis revealed that Jc C-INVD expressed differentlyin different tissues,with abundantly in cotyledon,but scarcely in root,and remarkably cold-induced expression in leaf.