中文摘要：

目的观察苯代谢物对K562细胞转铁蛋白受体表达的影响以及DNA甲基化抑制剂5-氮杂-2’-脱氧胞苷的干预作用。方法 K562细胞用苯代谢物处理72 h,处理期间的后48 h加入5-氮杂-2’-脱氧胞苷进行干预,然后利用荧光标记抗体结合流式细胞技术分析细胞表面转铁蛋白受体的表达,应用半定量反转录PCR技术分析细胞转铁蛋白受体mRNA的表达水平。结果苯代谢物苯酚（200-800μmol/L）、氢醌（10-40μmol/L）和1,2,4-苯三醇（5-20μmol/L）均能浓度和时间依赖性抑制转铁蛋白受体在细胞表面的表达和浓度依赖性抑制细胞内转铁蛋白受体mRNA表达;而5-氮杂-2’-脱氧胞苷可显著拮抗苯代谢物抑制转铁蛋白受体表达的作用。结论苯代谢物可抑制红系祖细胞转铁蛋白受体的表达,而DNA甲基化可能参与其中,转铁蛋白受体的表达抑制会影响细胞铁的摄入,从而影响血红素合成,继而抑制血红蛋白合成影响红系分化。

英文摘要：

Objective To investigate the effect of benzene metabolites on transferrin receptor expression in K562 cells,and the effect of DNA methyltransferase inhibitor,5-aza-2’-deoxycytidine,on the effect of benzene metabolites.Methods The protein expression of transferrin receptor on the surface of K562 cells was determined by flow cytometry after staining with FITC-conjugated anti-transferrin receptor antibody.The semiquantitative reverse transcription-PCR was used to determine the mRNA level of transferrin receptor in K562 cells.Results After K562 cells were treated with phenol（200 - 800 μmol/L）,hydroquinone（10 - 40 μmol/L） or 1,2,4-benzenetriol（5 -20 μmol/L） for 24,48 and 72 h,the protein level of transferrin receptor on the surface of K562 cells showed a concentration-and timedependent decrease,and each of benzene metabolites inhibited the mRNA expression of transferrin receptor in K562 cells with concentrationdependence.When K562 cells were exposed to phenol（800 μmol/L）,hydroquinone（40 μmol/L） or 1,2,4-benzenetriol（20 μmol/L）,the addition of 5-aza-2’-deoxycytidine attenuated benzene metabolites-induced decrease in protein and mRNA expression of transferrin receptor.Conclusion Benzene metabolites inhibited the expression of transferrin receptor,and DNA methylation might play a role in this process.The decrease in transferrin receptor on the cell surface could impact iron transport into the cells,finally inhibiting hemoglobin synthesis analysis.