中文摘要：

目的观察使用转人组织因子途径抑制物（TFPI）的猪骨髓间充质干细胞在体外异种移植凝血障碍模型中的抗凝血作用。方法使用SV40T慢病毒载体将猪骨髓间充质干细胞永生化，并在永生化的细胞内转入人TFPI基因。在人血浆、猪主动脉内皮细胞、人单核细胞共培养的异种移植凝血功能障碍细胞模型中检测转人TFPI的猪骨髓间充质干细胞对异种凝血功能的影响。结果在转染SV40T基因后，猪骨髓间充质干细胞被成功永生化，并具有和原代细胞相同的形态和细胞表型，转入人TFPI的猪骨髓间充质干细胞可以稳定表达人TFPI，并可以在体外细胞模型中有效缓解异种移植凝血功能障碍。结论转人TFPI的猪骨髓间充质干细胞可以有效缓解人组织因子激活导致的异种移植凝血功能障碍，具备在异种移植动物实验中进行应用的潜力。

英文摘要：

Objective To observe the reception of using pig bone marrow stromal cells （BMSCs） that were transfected with human tissue factor pathway inhibitor （TFPI） to resolve the dysregulation of coagulation after liver xenotransplantation. Methods Pig BMSCs were immortalized by transfection with lentivirus containing SV40T and then transfection with human TFPI. At last the cells were tested for their ability to inhibit clotting in a model by co-ineubation of human plasma, human monocytes and pig aortic endothelial ceils. Results After transfection with SV40T, pig BMSCs were immortalized and similar to primary cells. The immortalized pig BMSCs showed a stable TFPI expression after transfection with human TFPI by lentivirus. Moreover, they showed the potential of regulating coagulation dysregulation in vitro. Conclusion Pig BMSCs transfected with human TFPI could solve the regulation dysregulation caused by TF activation effectively, and have the potential of resolving coagulation dysregulation in liver xenotransplantation.