中文摘要：

目的建立一种新的复合式老年痴呆（AD）大鼠模型,用于AD及其治疗药物的研究。方法选用15月龄Wistar大鼠,随机分为4组：正常老年组,Aβ25~35模型组,IBO模型组,Aβ25~35＋IBO模型组。双侧基底前脑注射制备各种老年痴呆动物模型。通过水迷宫试验,脑组织病理切片银染色、刚果红染色,比较各老年痴呆模型的差异。结果在定位航行中,Aβ25~35＋IBO组潜伏期（92.4±11.6）s比IBO组（73.6±8.4）s,Aβ25~35组（71.7±9.2）s明显增加（P〈0.01）。各模型组大鼠大脑皮层都出现类老年斑病理改变和神经元纤维缠结,但以Aβ25~35＋IBO组最明显。结论Aβ25~35合并IBO制备的老年痴呆模型在一定程度上较传统模型更好地模拟AD的发病特点,可作为AD及其治疗药物研究的一种新模型。

英文摘要：

Objective To establish a novel complex rat model of Alzheimer＇s disease （AD） for research of AD and its treatment. Methods The normal 15 months old Wistar rat were randomly divided into normal, Aβ25-35 model, IBO model [ injection of basal nucleus of Meynert （BNM） with ibotenic acid （IBO）] and Aβ25-35 ＋ IBO （accumulation Aβ25-35 and the composition of IBO） model groups. The rat water-maze test and the pathological observation with silver and congo red staining of the hippocampus and cerebral cortex were carried out to evaluate the mouse model of AD. Results The latency period in positioning navigation test in Aβ25-35 ＋ IBO group [ （ 92. 4 ±11.6 ） s was significantly higher than that in Aβ25-35 [ （73.6 ±8.4） s] and IBO [ （71.7 ± 9. 2） s] groups （ P 〈 0. 01 ）. The senile plaques-like change and neurofibrillary tangles in cerebral cortex were saw in these model groups, especially in Aβ25-35 ＋ IBO group. Conclusions The animal AD model induced by Aβ25-35 and IBO injected into basal nucleus of Meynert simulates some characteristics of human AD to some extent, which may be used as a novel model to study AD and its drug treatment.