中文摘要：

网关向量广泛地被开发了便于基因在众多的种类克隆；然而，为多重有机体是兼容的一个通用系统正在缺乏。作为多种用途的表示向量， pCS2+ 基于脊梁的表示 plasmids 广泛地在哺乳动物 / 鸟的文化房间或 Xenopus/zebrafish 胚胎为送信人 RNA (mRNAs ) 或蛋白质的高级表示被使用。迄今为止，有为克隆系统的网关适用的 pCS2+ 脊梁的向量的一间套房还是无法获得的。这里，我们产生了一套网关目的地向量，作为 pGCS (在 pCS2+ 的网关的 plasmids ) 说出向量，它能被熔化到一种选择经常使用氨基终端或 carboxyl 终端标签包括 MYC，哈，旗帜，他的， GST，以及 eGFP 荧光灯 epitope。这的系统的产生将基于脊梁的网关目的地向量放 pCS2+ 与克隆途径的传统的消化结扎相比以高速度，精确性，和可靠性在 DNA 的 vitro 再结合允许。因此，我们的系统加速功能的蛋白质的生产，没有沉闷地把基因变成不同表达式向量，它能广泛地在脊椎动物有机体的一个大变化被表示。而且，我们经由非盈利的 Addgene Plasmid 仓库做对研究社区可得到的网关向量的这个系列。

英文摘要：

Gateway vectors have been extensively developed to facilitate gene cloning in numerous species; however, a universal system that is compatible for multiple organisms was lacking. As a multipurpose expression vector, pCS2＋ backbone-based expression plasmids are widely used for high-level expres- sion of messenger RNAs （mRNAs） or proteins in mammalian/avian culture cells or Xenopus/zebrafish embryos. To date, a suite of vectors with pCS2＋ backbone applicable for Gateway cloning system were unavailable yet. Here, we generated a set of Gateway destination vectors, named as pGCS （plas- raids of Gateway in pCS2＋） vectors, which can be fused to a choice of frequently used aminoor carboxyl-terminal tags, including MYC, HA, FLAG, His, GST, as well as eGFP fluorescent epitope. The systematic generation of this set of pCS2＋ backbone-based Gateway destination vectors allows for in vitro recombination of DNA with high speed, accuracy, and reliability compared with the traditional ＇digestion-ligation＇ cloning approach. Thus, our system accelerates the production of functional pro- teins, which could be widely expressed in a large variety of vertebrate organisms without tediously transferring genes into different expression vectors. Moreover, we make this series of Gateway vectors available to the research community via the non-profit Addgene Plasmid Repository.