为了快速、准确地再现球面参考光预放大数字全息图的三维信息,采用理论推导和实验验证相结合的方式,对等曲率物光和参考光预放大数字全息显微系统的相位畸变补偿问题进行了研究。推导了该系统的点扩展函数,进而获得了物体再现光场的复振幅及相位畸变表达式,利用估测的相位掩模对再现光场相位畸变预补偿后进行相位解包裹,结合自动相位补偿方法获得待测样品较为准确的相位分布。对人体血红细胞进行了实验研究,结果表明,利用相位掩模对再现光场进行预补偿,一方面可以避免相位解包裹的错误,另一方面还可以有效消除再现光场的二次相位畸变,以实现利用单次自动相位补偿方法获得光场正确的相位分布。
In order to fastly and accurately reconstruct the three-dimensional information of the hologram recorded by pre-magnification digital micro-holography, the solution of phase compensation in pre-magnification digital microholography with equal-cruvature object beam and reference beam is studied theoretically and experimentally. The point spread function is derived, then the expression of reconstructed light field amplitude and phase aberration are obtained, and by using phase mask to pre-compensate with the phase aberration fastly, the phase for the reconstructed light field is unwrapped. At last, combined with automatic phase compensation, the accurate phase distribution of the sample is obtrained. The red blood cells are researched experimentally. The result shows that phase mask to pre-compensate, not only can avoid the error in phase unwrapped, but also effectively eliminate the secondary phase distortion in the reproduction of light field, which aims to achieve the right phase distribution using single automatic phase compensation method.