中文摘要：

目的 探讨长链非编码RNA尿路上皮癌相关1（UCA1）在肝癌肝内转移患者肿瘤组织和血浆中的表达及临床意义。方法 收集2015年1月至2016年1月第二军医大学长海医院确诊为肝癌肝内转移并手术切除的20例患者的肿瘤组织和术前、术后血浆样本,20例肝癌患者的肿瘤组织和血浆样本,以及20例健康受试者的血浆样本。采用q PCR法测定肿瘤组织及血浆样本中UCA1 mRNA的表达,并分析UCA1 mRNA表达与Child-Pugh分级、原发灶肿瘤大小、年龄、性别、饮酒史等临床病理特征的关系。结果 肝癌肝内转移患者肿瘤组织与血浆中UCA1 mRNA表达均高于肝癌患者（P〈0.01）,术后血浆中UCA1 mRNA表达低于术前（P〈0.05）。肝癌肝内转移患者肿瘤组织UCA1 mRNA的表达与原发灶肿瘤大小（P=0.025）和Child-Pugh分级（P=0.006）有关。结论 血浆UCA1可能是肝癌转移诊断和病情监测的标志物,UCA1的表达水平与肝癌发展密切相关。

英文摘要：

Objective To explore the expression and clinical significance of the long non-coding RNA （lncRNA） urothelial carcinoma-associated 1 （UCA1） in the patients with intrahepatic metastasis of hepatocellular carcinoma （HCC）. Methods We collected the tumor tissues, pre-and post-operative plasma samples of 20 HCC intrahepatic metastasis patients undergoing resection, the tumor tissues and plasma samples of 20 HCC patients, and the plasma samples of 20 healthy peoples from Changhai Hospital of Second Military Medical University between Jan. 2015 and Jan. 2016. qPCR was used to determine the UCA1 mRNA expression in the tumor tissues and plasma samples. We analyzed the relationship of UCA1 mRNA expression with clinicopathological characteristics, including Child-Pugh grade, the size of primary tumor, age, gender and drinking history. Results The expressions of UCA1 mRNA in the tumor tissues and the plasma of the patients with intrahepatic metastasis of HCC were significantly higher than those of HCC patients （P〈0.01）. In addition, UCA1 mRNA expression was significantly decreased before surgery in the patients with intrahepatic metastasis of HCC compared with after surgery （P〈0.05）. Expression of UCA1 mRNA in tumor tissues of HCC intrahepatic metastasis patients was correlated with the size of primary tumor （P=0.025） and Child-Pugh grade （P=0.006）. Conclusion UCA1 may be a diagnostic and monitoring marker for metastasis of HCC; UCA1 expression is closely related to the development of HCC.