中文摘要：

目的 检测CD69＋CD4＋CD25-T细胞在自身免疫性胰腺炎（AIP）患者外周血中的表达情况,进一步分析其临床意义。方法 收集2014年9月～2016年12月长海医院血液科诊断的32例AIP患者与同期体检的32例健康个体外周血分别作为实验组与对照组。采用密度梯度离心法分离外周血单个核细胞（PBMCs）,用流式细胞术检测PBMCs中的CD69＋CD4＋CD25-T,以ELISA法检测血浆中细胞因子的表达状况。两组间变量的比较采用两独立样本t检验,两计量资料间的关系以Pearson相关系数表示。结果 实验组与对照组CD69＋CD4＋CD25-T表达水平为10.36%±3.68% vs 3.99%±1.45%,（t=9.110,P〈0.000 1）; TGF-β的表达水平为399.86±121.88 vs 143.87±56.22 pg/ml,（t=10.79,P〈0.000 1）,差异均具有统计学意义。实验组CD69＋CD4＋CD25-T细胞与TGF-β呈正相关（r=0.653,P〈0.001）,与IL-4,IFN-γ,IL-2呈负相关（r=-0.442,-0.567,-0.351,P〈0.05）,差异具有统计学意义。结论 CD69＋CD4＋CD25-T细胞可能参与了AIP的免疫病理过程,是临床诊断与治疗潜在的生物学指标。

英文摘要：

Objective To test the expression level of CD69＋CD4＋CD25-T cells in peripheral blood from patients with auto- immune pancreatitis,and further analyze its clinical significance. Methods Peripheral blood samples from 32 patients with AIP diagnosed in hematological department, Changhai Hospital and 32 health individuals examined at the same time were collected from September 2014 to December 2016, they were classified as experimental and control groups, separately. Peripheral blood mononuclear cells （PBMCs） was acquired by density gradient centrifugation, CD69 ＋ CD4 ＋ CD25-T cells in PBMCs were tested by flow cytometry, and the expression level of cytokines in plasm was by ELISA. The comparison of varies between the two groups was measured by two independent samples＇ t test. The relationship between the two measure- ment data was measured by pearson correlation coefficient. Results The expression levels of CD69＋CD4＋CD25-T in experimental and control groups were 10.36%±3.68% vs 3.99%±1.45% （t=9. 110,P〈0. 0001）. The expression level of TGF-β was 399. 864-121.88 vs 143.87±56.22 pg/ml （t= 10.79,P〈0. 000 1） ,both with statistical significance. The levels of CD69 ＋ CD4 ＋ CD25-T in experimental was positively correlated with TGF-β （r= 0. 653, P〈0. 001） and negatively with IL-4,IFN-γ,IL-2 （r=-0. 442,-0. 567,-0. 351,P〈0.05） and there was statistical significance. Conclusion CD69＋CD4 ＋CD25-T cells might involve the immunopathology of AIP and could be the potential biomarker for clinical diagnosis and therapy.