中文摘要：

采用PCR-变性梯度凝胶电泳技术（PCR-DGGE）分析比较了34株分离于环境和水产养殖动物体内的副溶血弧菌及标准株16S-23S rDNA间区（Intergenic Spacer Region, ISR）的多态性和亲缘关系.结果表明, 34株副溶血弧菌的ISR经PCR-DGGE电泳后均能分离出4―10条条带, 共计产生15个多态性位点.所有菌株聚为H、I、J、K四大簇, 株间遗传差异最大为株A18和A25, 遗传距离达到0.4.ISR PCR-DGGE方法为副溶血弧菌基因分型提供了一种新的方法.

英文摘要：

Using PCR-denaturing gradient gel electrophoresis （PCR-DGGE）, the polymorphism of 16S-23S rDNA intergenic spacer regions （ISR） from 34 Vibrio parahaemolyticus strains, including type strains and those isolated from environment and aquatic animals, were analyzed. The phylogenic relationships of these strains were also analyzed. The results showed that the ISRs could be separated into 4-10 different bands through PCR-DGGE. Thirty-four V. parahaemolyticus strains shared 15 polymorphic sites. Using MVSP, 34 strains were clustered into four groups, H, I, J, and K. Strains A18 and A25 had farthest genetic distance, with a value of 0.4. The present study suggested that ISR PCR-DGGE provided a new method for genotyping of V. parahaemolyticus.