采用现场采集样品及室内测试方法,对生长在某铬污染区内,距污染源10 m和100 m的芦苇叶片光合及抗氧化特征进行分析.结果显示,距污染源10 m处芦苇叶绿素含量低于100 m处,类胡萝卜素含量几乎一致,而污染区内两处芦苇光合色素含量均明显高于非污染区;污染区内芦苇叶片叶绿体吸收光谱、低温荧光发射光谱及叶绿素荧光参数与非污染区一致,表明污染区芦苇光合作用光反应能正常进行;SOD与CAT活性均明显高于非污染区,其中10 m处两种酶活性略高于100 m处;丙二醛含量与非污染区基本相同,说明污染区芦苇叶片未受到严重氧化损伤.
Soil around a mound of chromium slag in an abandoned chemical factory is seriously polluted by chromium. Phragmites australis L. is one of the few plants which can grow luxuriantly in that area. Therefore, photosynthetic and anti-oxidation characteristics of leaves of Phragmites australis L., 10 and 100 meters far from the mound, were investigated. The results showed that the content of chlorophyll a, b and carotenoid were much higher than those from unpolluted area. In polluted area, the contents of chlorophylls in leaves of Phragmites australis L. from 10 meters was lower than those from 100 meters, in which chlorophyll b decreased much more than chlorophyll a; however, the content of carotenoid was almost identical. The absorption spectrum, low temperature fluorescence emission spectrum of chloroplast and parameters of chlorophyll fluorescence showed almost no difference as those from unpolluted area, which suggested that the photosynthetic light reaction could work well under the stress of chromium. The activity of SOD doubled and that of CAT increased by 40% compared with those from unpolluted area, and the activities from 10 meters appeared a little higher than those from 100 meters. The content of MDA changed lit- tle, indicating that the leaves of Phragmites australis L. in the chromium polluted area were not further oxidatively damaged.