采用水培方法,在玉米幼苗进入三叶期后向培养液中加入不同浓度氯化镉(0、10^-6、10^-5、10^-4 mol·L^-1),胁迫不同时间(5、10、15 d)后对幼苗进行各项指标检测。结果表明,低浓度(10^-6 mol·L^-1)及中浓度(10^-5 mol·L^-1)镉胁迫对幼苗生长均有较强刺激作用,而中浓度胁迫对地上部分作用更明显;低浓度镉胁迫对根部及叶片抗氧化酶(SOD、POD、CAT及ASA-POD)活性影响较小,只是根部POD、ASA-POD活性明显升高,而中浓度胁迫对除SOD外其他抗氧化酶活性均有显著促进作用;MDA含量均未有显著升高,可见根部及叶片未受到氧化损伤;两种浓度胁迫下,Fv′/Fm′虽下降,净光合速率有所升高。高浓度镉(10-4 mol·L^-1)胁迫下幼苗生长几乎停止;根部SOD及CAT活性升高,而POD和ASA-POD被明显抑制,叶片中CAT、POD及ASA-POD活性显著升高;根部受到显著氧化损伤;各项光合参数(Fv/Fm、Fv′/Fm′和净光合速率)均显著降低。
The influence of Cd^2+ to the antioxidant system and photosynthesis of maize( Zea mays L. ) was studied systematically. Seedlings were cultivated in Hoagland liquid medium, and in the three-leave-period were treated with different concentrations of CdC12 (0, 10^-6, 10^-5, 10^-4 mol· L^-1 ). After 5,10 and 15 days of stress, physiological parameters of seedlings were measured. In the stress of low( 10^-6 mol· L^-1 ) and middle( 10^-5 mol·L^-1 ) concentration, the growth of seedlings was promoted obviously whereas that of parts above the earth more notably in the stress of 10^-5 mol·L^-1; In the stress of 10^-6 mol·L^-1, the activities of POD and ASA-POD in root increased and activities of other anti-oxidant enzymes changed little; However, the stress of middle concentration made activities of anti-oxidant enzymes increase obviously except SOD; The content of MDA was not high compared with control, which showed that seedlings were not hurt by oxidation; Fv′/Fm′ decreased and the net photosynthetic rate rose. In the concentration of 10^-4 mol· L^-1, the seedlings almost did not grow; The activities of SOD and CAT in root increased and those of POD and ASA-POD were inhibited seriously, and the activities of CAT, POD and ASA-POD in leave increased sharply; Root was hurt oxidately; Photosynthetic parameters(Fv/Fm, Fv ′/Fm′ and net photosynthetic rate ) decreased notably.