中文摘要：

背景：器官移植后如何监测受者的免疫状态,并预测排斥反应的发生,从而调整免疫抑制剂的用量是当前面临的重要问题。目的：探索新型免疫状态定量监测方法在小鼠皮肤移植模型中是否有抗原特异性。方法：建立C57→BALB/c皮肤移植排斥模型,分别输注C57/BALB/c混合脾细胞悬液及第三者对照DBA/BALB/c混合脾细胞,在细胞输注后检测2种混合细胞悬液比例变化及供体细胞杀伤率,同时设置BALB/c→BALB/c同系皮肤移植对照组,免疫低下裸鼠对照组及免疫抑制剂对照组与之对比。结果与结论：C57/BALB/c悬液-移植排斥模型组和C57/BALB/c悬液-移植排斥模型免疫抑制剂组小鼠对供体C57脾细胞有强烈的特异性杀伤作用,其中C57/BALB/c悬液-移植排斥模型免疫抑制剂组特异性杀伤作用稍弱,而输注第三者对照DBA/BALB/c混合脾细胞的小鼠细胞注射2～4h内没有明显特异杀伤作用,但免疫低下的裸鼠始终未表现出特异性杀伤。说明实验建立的抗原特异性免疫状态检测方法能够快速地检测出皮肤移植受体的免疫状态。

英文摘要：

BACKGROUND：How to monitor the recipient＇s immune status,and predict the occurrence of rejection after organ transplantation,in order to adjust the dosage of immunosuppressive agents is the important issue faced currently.OBJECTIVE：To investigate antigen-specificity of new quantitative monitoring of immune status in skin transplantation model rats METHODS：The C57→BALB/c skin graft injection model was established,the models were injected with C57/BALB/c mixed splenocytes suspension and third party DBA/BALB/c mixed splenocytes suspension respectively.The proportion of mixed cell suspension and killing rate of donor cells were detected after cells injection.The homologous series skin transplantation control group was setted,and compared with immunocompromised nude mice control group and immunosuppressant control group.RESULTS AND CONCLUSION：The C57/BALB/c suspension in model group and C57/BALB/c in immunosuppressant group had a strong specific cytotoxicity on C57 splenocytes of the donors.The specific cytotoxicity of C57/BALB/c suspension in immunosuppressant group was weak,and there was no obvious specific cytotoxicity in the cells at 2-4 hours after third party DBA/BALB/c mixed splenocytes injection.However,the cells of immunocompromised nude mice had no specific cytotoxicity.The antigen-specific immune status detection method established in the experiment can detect the immune status of skin transplant recipients rapidly.