中文摘要：

通过化学分析和生物活性评价考察丹参药材的品质差异,探讨丹参抗血小板聚集生物活性的主要贡献成分。采用高效液相色谱（HPLC）技术建立丹参药材HPLC指纹图谱,以抗血小板聚集相对效价作为指标,评价不同产地不同批次丹参药材的品质差异,构建基于化学表征及生物效价测定的评价模式。结果表明,不同批次丹参药材的HPLC指纹图谱相似度很高（相似度0.930~0.998）,而其抗血小板聚集相对效价相差10倍,提示化学指纹图谱难以反映丹参的活性和质量差异。通过化学指纹图谱与抗血小板聚集生物效价进行谱效相关分析,筛选出与生物活性相关系数大于0.5的6个色谱峰：二氢丹参酮Ⅰ、隐丹参酮、丹参酮Ⅰ、丹参酮ⅡA及2个未知化合物。对上述4种已知化合物单体进行活性验证发现,隐丹参酮的抗血小板聚集活性最强,而其它3种丹参酮类化合物几乎没有体外抗血小板聚集活性。进一步比较丹参中高含量成分丹酚酸B与低含量成分隐丹参酮的活性贡献,结果表明,两者的活性贡献基本相当,说明隐丹参酮是丹参中低含量高活性成分,对评价丹参质量具有重要贡献度。

英文摘要：

Chemical analysis and bioass ay we r e u s e d to d i s t in g u i s h q u a l i ty g r a d e s o f S a lv ia m i l t io r r h i z a b g e . , and the active compounds were invest igat ed o n a nt i - pl a t e l e t a g g r e g a t io n . We e s t a b l i s h e d th e H P L C f in g e r p r in t of Salvia miltiorrhiza bge . and meansur ed t hi r b io p o t e n c y b a s e d o n a n t i - p l a t e l e t a g g r e g a t io n . T h e r e s u l t s showed that the HPLC fingerprints of di f f er ent ba t c he s o f S a lv ia mi l t io r r h i z a b g e . w e r e s im i l a r （ S im i la r i ty = 0.930-0.998） , but the di fference of biopot ency c o u ld b e t e n - f o l d . T h ro u g h c o r r e la t io n a n a ly s i s o f c h em i c a l fingerprint and anti-platelet aggr egat ion assay,dihydr otans hinone I ,c r y p t o t a n s h i n o n e ,t a n s h in o n e I , tanshinone 域 A , and two unknown c ompounds we r e c lo s e ly r e l a t e d to t h e b i o p o t e n c y . I t w a s p r o v e d th a t cryptotanshinone was a high act ivi ty component o n p l a t e l e t a g g r e g a t io n . T o c o m p a r e th e a c t iv e c o n t r ib u t io n o f the high content Salvianolic acid B and low c ont e nt o f th e a c t iv e in g r e d i e n t c r y p t o t a n s h i n o n e, th e a c t iv e contribution value of the two compounds we r e b a s i c a l ly e q u i v a l e n t. C r y p t o t a n s h i n o n e ,w h ic h h a d low c o n t e n t but high activity, made an impor t ant cont r ibut ion to e v a lu a t in g th e q u a l i ty o f S a lv ia m i l t io r r h i z a b g e . . T h e results indicated that the qual i ty of t r adi t ional Ch in e s e me d i c in e c o u ld n o t b e e v a lu a t e d a c c u r a t e ly b y o n ly using the chemical content or abundance . Thi s wo rk e s t a b l i s h e d a q u a l i ty e v a lu a t io n m o d e l b a s e d o n c h em i c a l fingerprint