中文摘要：

目的建立稳定转染ndrg2基因的脑胶质瘤细胞系，以探索及研究该基因的功能。方法以低表达ndrg2基因的脑胶质瘤细胞系U251为材料，以ndrg2基因转染U251细胞系，经G418进行筛选后，挑取单克隆进行培养，用荧光显微镜观察、逆转录酶聚合酶链反应（RT—PCR）验证获得的表达细胞株。结果经转染和G418筛选后，荧光显微镜下见有明显表达ndrg2基因的细胞约占70％-80％，RT-PCR检测到转染基因ndrg2后细胞ndrg2的高表达，而转染空载体的细胞ndrg2低表达。结论成功建立了稳定转染基因ndrg2的脑胶质瘤细胞系U251，为进一步探讨该基因的功能奠定了一定基础。

英文摘要：

Objective To establish a glioma cell line with ndrg2 gene steady transfection in vitro. Methods A glioma cell line U251 with low expression of ndrg2 gene was transfected with ndrg2 gene （pEGFP-c2-ndrg2）. After screened with G418, the single cell clone was sought out and cultured. Expression of ndrg2 gene was detected by reverse transcription polymerase chain reaction （RT-PCR） and fluorescene microscope. Results After tansfected with ndrg2 gene and screened with G418, the glioma cells with high expression of ndrg2 gene accounted for 70% ~ 80% of all the cells under fluorescene microscope. High expression of ndrg2 gene was detected by RT-PCR, while the expression of ndrg2 gene of the cells transfected with blank vector （pEGFP-c2） was low. Conclusion A glioma cell line with ndrg2 gene steady transfection is established successfully, which paves way for the further study of the function of ndrg2.