中文摘要：

实验以鸡肉粉∶牛肉骨粉∶喷雾血球干燥粉∶水解羽毛粉=40∶35∶20∶5的比例组成的混合动物蛋白（animal protein blend,APB）分别直接替代或在补充赖氨酸、蛋氨酸、苏氨酸后替代花鲈饲料中50%、75%、100%的低温干燥鱼粉,研究其对花鲈生长及生理功能的影响。经过8周的饲养后发现,花鲈的生长性能随替代比例的提高而降低;摄食含APB饲料的花鲈,其成活率,摄食率和生长性能均显著低于对照组（P〈0.05）。与APB直接替代鱼粉的处理组相比,添加3种必需晶体氨基酸极显著提高了花鲈的生长性能及全鱼蛋白、脂肪、能量含量（P〈0.01）,显著降低了花鲈的全鱼水分、灰分,肝指数及脏体比（P〈0.05）。晶体氨基酸与APB的替代水平对花鲈的生长性能、形体指标及体组成成分存在显著的交互作用（P〈0.05）。实验结果表明,（1）混合动物蛋白替代初始体重为13.2 g的花鲈饲料中白鱼粉的比例应小于50%;（2）添加必需晶体氨基酸能显著提高APB在花鲈饲料中的应用潜力;（3）花鲈具备一定的利用晶体氨基酸的能力。

英文摘要：

An 8-week growth trial was conducted to evaluate the effects of an animal protein blend（APB）with poultry by-product meal,meat and bone meal（beef）,spray dried blood meal,and hydrolyzed feather meal at the ratio of 40∶ 35∶ 20∶ 5 to replace low temperature steam-dried fish meal with or without supplementations of lysine,methionine and threonine in diet of juvenile Japanese sea bass,Lateolabrax japonicus,on growth performance and body composition.Three replacing levels were designed at 50%,75% and 100%,respectively.Growth performance of fish was reduced with higher APB inclusion levels;Fish fed control diet showed significantly higher survival feeding rate（FR）,specific growth rate（SGR）and protein efficiency ratio（PER）than those fed diets containing levels of APB;Supplementation of crystallized amino acids（CAA）enhanced the feeding rate and growth performance and reduced hepatosomatic index and increased whole body crude protein,crude lipid and gross energy.Interactions of CAA supplementation and fishmeal replacing levels on growth performance and body composition were significant.Results demonstrate that 1） no more than 50% of LT-FM can be replaced by APB in diet of juvenile Japanese sea bass;2） supplementations of CAA could improve potential of APB replacing dietary fish meals;3） crystallized amino acids can be relatively efficiently utilized by Japanese sea bass.