中文摘要：

目的探讨转染血管内皮生长因子（VEGF）基因的小鼠NIH3T3细胞移植对缺血皮瓣的血管新生和皮瓣存活率的影响。方法体外PcDNA3．1（-）／VEGF165质粒转染小鼠NIH3T3细胞，免疫组化方法检测小鼠NIH3T3细胞体外表达VEGF的情况，CM-DiI标记小鼠NIH3T3细胞。将小鼠随机分为3组：A组[PcDNA3．1（-）／VEGF165质粒转染的NIH3T3细胞移植]、B组（单纯NIH3T3细胞移植）、C组（单纯DMEM培养基注射）。每只小鼠背侧皮下按组分别注射细胞悬液和培养基，注射后酶联免疫吸附（ELISA）法连续检测大鼠血浆VEGF浓度，注射后第4天掀起一个蒂在尾侧的4．0cm×1．5cm的随意皮瓣。术后第7天分别观察皮瓣的存活率、血流灌注、皮瓣毛细血管密度、NIH3T3细胞在皮瓣内的分布和存活情况。结果转染VEGF165基因的小鼠NIH3T3细胞体外和体内检测均高表达VEGF165蛋白。A组的皮瓣存活率、毛细血管密度、血流灌注比值均显著高于另外两组（P〈0．05）。结论转染VEGF基因的小鼠NIH3T3细胞皮下移植可促进缺血皮瓣的血管新生，提高存活率。

英文摘要：

Objective To investigate the feasibility of applying NIH3T3 cells transfected by VEGF gene to the treatment of ischemic random skin flaps. Methods Plasmid PcDNA3. 1 （ - ）/VEGF165 containing VEGF gene was transduced into the mouse NIH3T3 cells by liposome. Immunohistochemistry was used to detect the expression of VEGF protein of mouse NIH/3T3 cells in vitro. The NIH3T3 cells were stained with CM-DiI before the transplantation. Thirty mice were randomized into 3 groups： Groups A, B and C, and were respectively injected with NIH/3T3 cells tansfected with PeDNA3. 1 （ - ）/VEGF165 plasmid, NIH/3T3 cells and medium only. On the 4th day after the injection, random dorsal skin flaps with an area of 4. 0 cm × 1.5 cm were established. The survival, neovasculariztion and blood flow recovery of the flaps were detected. Results VEGF-transduced NIH3T3 cells expressed VEGF highly in vitro and in vivo. The results showed that flap survival rate in group A （ 95. 1% ± 3. 1% ） was significantly higher than those in group B （37. 4% ± 6. 3% ） and group C （26. 2% ± 5.6% ）. The capillary density and the blood perfusion of the flaps in group A were significantly higher than those in other two groups. Condusions VEGF- transfected NIH3T3 cells can improve ischemic flap neovascularization and extend survival areas.