中文摘要：

方剂是中医临床用药的主要形式、中医经验的载体,其配伍规、组方原则是中医药理论的精华。方剂药理研究是现代中医药研究的重点也是难点。由于病因、病机、中药药味及其组合方式的多样性,使得方剂的研究难度很大。结合现代科学技术对其加以研究对推进中医药理论现代化、科学化、创新发展具有重要意义。在运用现代药理学技术对方剂加以研究分析时应注重,坚持以中医理论为指导,结合运用现代系统论思想,采用现代科学手段,由简到繁,由宏观到微观,促使药理学与中医理论科学结合,从而全面揭示中药方剂防病治病的理论基础与作用机制。近年来对于方剂药理研究的方法主要集中在以下几个方面：全方研究、拆方研究、同类方剂对比研究、方剂剂型研究、煎服法研究、药代动力学研究。同时将方剂药理研究与生物化学、药物化学、分子生物学、中药血清药物化学、复方代谢组学、基因组学及蛋白质组学等现代科学技术相结合,有效的推进了方剂学发展。各种研究方法各有其适用范围及优势,但有时也存在一些不足,如中医整体观念、现代技术研究脱离中医理论存在局限性,整体与局部脱节,宏观与微观脱节,体内过程与活性评价脱节等,因此通过对以上实验研究方法加以探讨总结,明确中药方剂现代化研究的思路以期促进方剂研究更加合理有效的与现代科学技术相融合,避免局限性,促进方剂药理研究创新性发展。

英文摘要：

Prescriptions are the main clinical application form of traditional Chinese medicine （TCM） , carrier of TCM, and its compatibility regularity and formulation principle are the essences of TCM theories. Pharmacological research of prescriptions is the key point of modern TCM study. Its difficulty is based on the various pathogenesis and TCM combinations. Modern scientific technology could significantly promote the modernization, scientific and innovative development of TCM theories. Modern studies on TCM should focus on TCM theories, and be integrated with modern system theories and technological methods, so as to reveal the mechanism of TCM prescriptions for preventing and treating disease theories in an all-round way. In recent years, pharmacological research methods are mainly concentrated in the following aspects, namely whole prescription study, decomposed prescription study, comparative study on similar prescriptions, dosage form study, decoction method study and pharmacokinetic study. The pharmacology methods are combined with modem techniques, such as biochemistry, medicinal chemistry, molecular chemistry, serum pharmacochemistry of TCM, prescription metabonomics, genomics and proteomics, so as to promote the prescription development significantly. Each methodhas its own application range and advantages, but some shortages, such as holistic TCM concept deficiency, separation between whole and parts, separation between macroscopic and microscopic views, and methods limitations. Therefore, we make conclusions on the above methods, in order to make good combination on prescriptions and modern techniques, avoid limitations and promote pharmacological research development.