中文摘要：

目的：利用免疫组化法研究温胆汤、化瘀温胆汤对糖耐量低减（ IGT ）大鼠脂肪中PI3 K、PKB、GSK－3β蛋白表达的影响。方法：40只SD大鼠随机分为空白对照组、IGT模型组、温胆汤组和化瘀温胆汤组，喂养24周后，取脂肪采用免疫组化法测定PI3 K、PKB、GSK－3β蛋白含量。结果：与空白对照组比较，IGT组PI3K、PKB蛋白含量显著降低（P＜0．05），GSK－3β蛋白含量显著升高（P＜0．05）。与IGT组比较，温胆汤、化瘀温胆汤均可显著升高PI3 K、PKB蛋白含量（ P＜0．05），降低GSK－3β蛋白含量（ P＜0．05）。结论：温胆汤、化瘀温胆汤可以不同程度的作用于IGT大鼠脂肪的PI3K通路。

英文摘要：

Objective：The experiment aimed to study the protein expressions of phosphatidyl inositol 3-kinase（PI3K）, protein kinase B（ PKB） and glycogen synthase kinase-3β（ GSK-3β） in the tats′fat by immunohistochemistry method. Methods：The SD rats were divided into four groups at random,which were blank control group,IGT model group,Wen-dan decoction group and Huayu Wendan decoction group.Detect the protein expressions of PI3K,PKB and GSK-3βin the fat by immunohistochemical staining.Results：Compared with the blank control group, the protein expressions of PI3K and PKB in fat of IGT group all decreased,but the protein expression of GSK-3βincreased obviously.Conclusion：Wendan decoction and Huayu Wendan decoction can have an effect on different levels of PI3K pathway in the IGT rats.