中文摘要：

目的：阐述LHA和PVN间nesfatin-1神经通路的构成,探讨PVN nesfatin-1对胃收缩幅度及频率的影响及潜在机制.方法：采用荧光金逆行追踪结合荧光免疫组化技术,观察LHA-PVN间nesfatin-1神经通路构成;采用细胞外放电记录,观察nesfatin-1对GD放点活动的影响;通过在体胃运动技术,观察nesfatin-1对清醒自由活动大鼠胃收缩幅度和频率的影响.结果：Nesfatin-1能够抑制GD-E神经元放电（1.97± 0.12Hzvs.1.15± 0.07 Hz）促进GD-I神经元放电（1.74± 0.10 Hz vs.3.04± 0.18 Hz）,H4928能够部分阻断nesfatin-1对GD神经元（GD-E：1.38± 0.08 Hz,P〈0.05 vs.nesfatin-1;GD-I：2.49± 0.15 Hz,P〈0.05 vs.nesfatin-1）的影响;PVN内微量注射nesfatin-1能够抑制大鼠胃运动,呈量效依赖关系;LHA和PVN间有nesfatin-1神经纤维联系;电刺激LHA后,GD神经元放电频率增加（GD-E：2.06± 0.12 Hz vs.4.23±0.21Hz,GD-I：1.61±0.09 Hz vs.4.83± 0.25 Hz）,预先向PVN注射抗NUCB2/nesfatin-1抗体后,GD-E神经元放电频率减弱（4.91±0.25 Hz vs.4.23±0.21 Hz）,而GD-I神经元放电频率增强（4.15±0.18 Hz vs.4.83±0.25）.结论：PVN内nesfatin-1可调控大鼠GD神经元放电活动及胃运动,该效应受LHA调控.

英文摘要：

Objective：The current study investigated the effects of nesfatin-1 in the hypothalamic paraventricular nucleus （PVN） on gastric motility and the regulation of the lateral hypothalamic area （LHA）.Methods：The projection of nerve ？ber and expression of nesfatin-1 were observed by retrograde tracing and fluo-immunohistochemistry staining;The nuclei microinjection and nuclei electrical stimulation,extracellular discharges of single unit neuron were used to observe the effects of nesfatin-1 on the GD neurons;Gastric motility recording in vivo were used to monitor the effects of nesfatin-1 on the amplitude of constriction and frequency of gastric motility in conscious rats.Results：Nesfatin-1 inhibited the majority of the GD-E neurons（1.97± 0.12 Hz vs.1.15± 0.07 Hz） and excited GD-I neurons （1.74± 0.10 Hz vs.3.04± 0.18 Hz） in the PVN,which were weakened by oxytocin receptor antagonist H4928 （GD-E：1.38± 0.08 Hz,P〈0.05 vs.nesfatin-1;GD-I：2.49± 0.15 Hz,P〈0.05 vs.nesfatin-1）.Gastric motility experiments showed that administration ofnesfatin-1 in the PVN decreased gastric motility.Retrograde tracing and immunofluorescent staining showed that nucleobindin-2/nesfatin-1 and fluorogold double-labeled neurons were observed in the LHA.Electrical LHA stimulation excited the firing rate of GD-responsive neurons （GD-E：2.06± 0.12 Hz vs.4.23± 0.21 Hz,GD-I：1.61± 0.09 Hz vs.4.83± 0.25 Hz） in the PVN.Pre-administration of an antinucleobindin-2/nesfatin-1 antibody in the PVN strengthened gastric motility,decreased GD-E neurons （1.74± 0.10 Hz vs.3.04± 0.18 Hz） and excited the discharging of the GD-I neurons（4.15± 0.18 Hz vs.4.83± 0.25） induced by electrical stimulation of the LHA.Conclusion：Nesfatin-1 in the PVN could serve as an inhibitory factor to inhibit gastric motility,which might be regulated by the LHA.