植物-微生物联合修复是一种颇具潜力的治理重金属污染土壤的方法,筛选合适的微生物提高植物对重金属的吸收是该修复方法的重要内容。采集株洲市清水塘工业区株洲冶炼厂附近土壤作为研究对象(采样深度为0~20cm),采用稀释培养法和CAS检测平板分离筛选产铁载体的抗镉微生物;通过对目的菌种镉离子最低抑茵浓度的测定和显微镜及扫描电镜(SEM)对其形态的观察,实验结果表明细菌x19,x22和真菌Z1能分泌铁载体,且对镉产生较强的抗耐性,根据CAS变色程度初步断定其分泌铁载体的能力为Z1〉x19〉X22;菌株X19,X22和z1的镉离子最低抑菌质量浓度(MIC)分别为450~220,800mg/L。
The Plant-Microbial Remediation is a potential method for the disposal of heavy metal polluted soils, and the selection of appropriate microorganisms to improve the plant absorbability of heavy metal is important for the mainte- nance method. With the soil of Zhuzhou Qingshuitang industrial zone as the study object (sampling depth is 0 to 20 cm), the strains which can secrete siderophore and resist cadmium in the samples were screened by the dilution culture method and the CAS method. Minimum inhibitory concentration of cadmium ion in the objective strain was determined and its morphol- ogy was observed through the microscope and scanning electron microscope (SEM). The result showed that the strains of X19(bacteria), X22 ( bacteria ) and ZI ( fungi ) can secrete siderophore and has strong resistance to cadmium, MICs of strains X 19, X22 and Z1 to cadmium are 450mg/L,220 mg/L and 800mg/L respectively, and the ability of secreting siderophore was Z1 〉 X19〉 X22, judged by CAS color variation.