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产铁载体菌的筛选鉴定及活化镉的性能探究
  • ISSN号：1003-6504
  • 期刊名称：《环境科学与技术》
  • 时间：0
  • 分类：X172[环境科学与工程—环境科学]
  • 作者机构：[1]湖南工业大学绿色包装与生物纳米技术实验室,湖南株洲412007, [2]银丰生物工程集团有限公司,山东济南250000
  • 相关基金：国家自然科学基金项目资助（31100082,61171061）; 湖南省自然科学基金项目资助（14JJ2149）; 株洲市重大科技专项基金项目资助（11ZZKJ-03）; 湖南工业大学自科基金项目资助（2011HZX02）; 湖南工业大学研究生创新基金项目资助（CX1205）; 湖南省科技厅科技计划项目资助（2012SK4028）
作者： 魏本杰[1], 曾晓希[1], 刘志成[2], 杨少迪[1], 朱生翠[1], 汤建新[1]
关键词： 镉活化菌, 铁载体, 抗镉微生物, 16S, rDNA鉴定, 荧光检测, Cd-mobilization bacteria, siderophores, cadmium-resistant strain, 16S rDNA identification, fluorescence intensity
中文摘要：

从湘江流域某冶炼厂周边土壤中筛选出高抗镉微生物,在镉离子浓度为2 mmol/L的培养基中正常生长。通过改进的CAS平板上检测出2株具有铁载体产生能力的细菌H1和H2。H1产物具有较强的荧光性能,而且H1菌对重金属Cd有较强的活化能力。接种4 d后,土壤中可溶性镉的含量（21 mg/kg）是对照组（10.4 mg/kg）的2倍多。用扫描电子显微镜观察显示其形态与假单胞菌类似;其16S rDNA序列与Pseudomonas sp.序列相似度达99%。

英文摘要：

The cadmium-resistant（Cd） strains which can normal grow in the concentration of 2 mmol/L Cd medium were isolated from metal contaminated soil around a smeltery beside Xiangjiang River in China. Then sidorophores-producing bacteria（SPB） H1 and H2were screened out using the modified Chrome Azurol S（CAS）-agar plate. The product of H1 with strong fluorescence intensity can mobilize Cd in soils. After 4 d vaccination, the concentration of soluble cadmium with H1（21mg/kg） was more than twice as higher than the control group（10.4 mg/kg）. H1 strains have the same morphology with Pseudomonas, and 16 S rDNA sequence has 99% similarity to Pseudomonas sp.

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期刊信息
  • 《环境科学与技术》
  • 中国科技核心期刊
  • 主管单位:湖北省环境保护厅
  • 主办单位:湖北省环境科学研究院
  • 主编：袁道先
  • 地址：武汉市武昌珞珈山八一路338号
  • 邮编：430072
  • 邮箱：hjkxyjs@yahoo.com.cn
  • 电话：027-87643502 87643503
  • 国际标准刊号：ISSN：1003-6504
  • 国内统一刊号：ISSN：42-1245/X
  • 邮发代号:38-86
  • 获奖情况:
  • 中文核心期刊,第三界国家期刊奖湖北省科技期刊参评提名奖,全国环境期刊一等奖
  • 国内外数据库收录:
  • 美国化学文摘（网络版）,英国农业与生物科学研究中心文摘,波兰哥白尼索引,美国剑桥科学文摘,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊（2004版）,中国北大核心期刊（2008版）,中国北大核心期刊（2011版）,中国北大核心期刊（2014版）
  • 被引量:37319