中文摘要：

目的观察大剂量60 Coγ射线照射恒河猴后造血系统相关指标的早期改变,为急性放射病（ARS）早期造血因子治疗提供实验依据。方法 33只雄性成年恒河猴随机分为对照组和不同照射剂量的照射组,照射组动物用60 Coγ射线4、8和12Gy一次全身照射建立ARS恒河猴模型。XE-2100全自动血细胞分析仪检测照射前和照射后3、6、9、12、24、48、80h血常规指标,照射后6、48和80h麻醉处死动物,取胸骨观察病理组织学变化。结果 4、8Gy 2个剂量照射后3h外周血白细胞均低于照射前检测值,照射后6h白细胞均升高,9h检测值最高,分别达136.04%和221.38%（以照射前检测值为100.00%,下同）,12h后开始明显下降。而12Gy照射后白细胞持续升高,9h达到最大值,之后下降,均显示出明显的＂暂时性回升＂峰。照射后中性粒细胞数先升高,9h达高峰之后又快速下降;照射后外周血淋巴细胞数急剧下降,3h检测值仅为照射前的12.02%~25.04%。照射后胸骨骨髓有核细胞数目急剧减少,照射剂量越大,残存造血细胞数越少。结论骨髓型ARS早期外周血白细胞和中性粒细胞数＂暂时性回升＂的时间节点可视为造血因子治疗的最佳给药时机。

英文摘要：

Objective To observe the early changes of related indexes after high dose of 60 Coγ-ray irradiation on rhesus monkey hematopoietic system.Methods A total of 33 rhesus monkeys were randomly divided into normal control and different irradiation control group,rhesus monkeys in irradiation control group were given different doses（4,8,12Gy）irradiation to establish acute radiation sickness（ARS）models.XE-2100 automatic blood cell analyzer detected the peripheral blood before and after the irradiation of 3,6,9,12,24,48,80 h.The rhesus monkeys were sacrificed to have a observation of sternum pathological changes at 6,48 and 80h after 4,8,12 Gy 60 Coγ-ray irradiation.Results The number of white blood cell in peripheral blood of the rhesus monkeys after 4and 8Gy 60 Coγ-ray irradiation were lower than that before irradiation at 3hafter irradiation,as was significant increased at 6hafter irradiation,the highest values were 136.04%.and 221.38% after 9h（with before irradiation values was 100.00%,the same below）,become obviously drooped from 12 hafter irradiation,show clearly ＂temporary peak＂.But the number of white blood cell after12 Gy 60 Coγ-ray irradiation was significant increased at 6hafter irradiation,at the highest of 9h,become obviously drooped from12 hafter irradiation.Peripheral blood neutrophile count was significant increased at 6hafter irradiation,at the highest of 9h,become obviously drooped from 12 hafter irradiation.Peripheral blood lymphocyte count fell sharply after irradiation,3hdetection value was only 12.02%-25.04% of before irradiation.Sternal bone marrow nucleated cell number decreased sharply after irradiation,the more irradiation dose,the less residual hematopoietic cells.Conclusion In the early stage of BM-ARS, ＂temporary peak＂ time node of the white blood cell and neutrophil count could be regarded as the best delivery time of hematopoietic cytokine therapy.