采用盆栽试验研究了螯合剂EDTA 和柠檬酸(CA)单施或配施对蓖麻(Ricinus communis)生理特性及修复Cd 污染土壤能力的影响.结果表明:单施EDTA 显著降低蓖麻地上生物量和叶绿素含量(P〈0. 05),显著影响蓖麻叶片抗氧化酶活性(P〈0. 05),10 mmol·kg^-1 EDTA 甚至抑制SOD 和POD 活性;CA 与EDTA 联用可在-定程度上缓解EDTA 对蓖麻的毒害作用,CA 可缓解1 和5 mmol·kg^-1 EDTA 导致的蓖麻地上生物量和叶绿素含量降低,缓解1 mmol·kg^-1 EDTA 导致的MDA 含量升高;单施5 mmol·kg^-1 EDTA 蓖麻对Cd 的富集和转移能力最高,但单株Cd 富集量未达最大值,而2 mmol·kg^-1 CA 与5 mmol·kg^-1 EDTA 配施却使蓖麻单株总Cd 富集量和提取率达到最高,分别是对照的2. 98 和3. 00 倍.因此,CA 与EDTA 联用在强化蓖麻修复Cd 污染土壤中具有很好的应用潜力.
A pot experiment was conducted to study effects of citric acid ( CA) and EDTA on physiological characteristics and Cd accumulation of R. communis. Results showed that the application of EDTA alone significantly reduced the biomass of shoots and the content of chlorophyll of R. communis, evidently affected the activity of antioxidant enzyme in leaves of the plant(P<0?05), and even inhibited the activities of SOD and POD, when the application rate reached 10 mmol· kg-1 . The application of EDTA and CA together reduced the toxicity of EDTA to R. communis to a certain extent, the effect of EDTA, at 1 or 5 mmol·kg-1 , lowering the biomass of shoots and the content of chlorophyll of R. communis and the effect of EDTA, at 1 mmol·kg-1 , increasing the content of MDA in the plant. The application of EDTA alone, at 5 mmol·kg-1 , brought Cd accumulation and transfer capacity of the plant to the peak. However, once 2 mmol·kg-1 CA was applied in combination, the effect of EDTA reached a new peak, 3 times as high as in CK. Therefore, combined application of EDTA with CA should be a practice of great potential in enhancing the effect of R. communis remedying Cd polluted soils.