中文摘要：

目的探讨帕罗西汀对星形胶质细胞细胞增殖的作用及其相关机制。方法原代培养海马星形胶质细胞．随机分为4个研究组和对照组（sham组）。研究组细胞分别直接给予终浓度为5、10、20和50μmoL／L剂量的帕罗西汀，处理24或48h。采用细胞计数试剂盒（CCK-8）检测星型胶质细胞活性，免疫细胞化学观察细胞形态学改变，5-溴脱氧尿嘧啶核苷（Brdu）检测细胞增殖情况，蛋白质印迹法（Western blotting）检测磷酸化细胞外信号调节激酶（phosphorylatged extraeellular single—regulated kinase，pERK1／2）的表达水平。结果48h后各组的星形胶质细胞活力、BrdU阳性细胞数量以及pERKI／2的表达差异均有统计学意义（P〈0．001），进一步两两比较．10μmoL／L帕罗西汀组的上述3个指标均高于其他剂量组和对照组（P〈0．01）：而50μmoL／L帕罗西汀组细胞活力均低于其他组（P〈0．01）。5、10和20μmoL／L帕罗西汀组作用24或48h后，细胞活力、BrdU阳性细胞数量以及pERK1／2的表达与对照组之间无差异（P〉0．05）。结论帕罗西汀能够促进星形胶质细胞的活性和增殖,而且这种作用可能与ERK信号通路有关。

英文摘要：

Objective To investigate the effect of paroxefine on the proliferation of hippocampus-derived astrocytes, and explore its potential molecular mechanism. Methods Astrocytes were isolated from the hippocampus of fetal rats and were treated with different concentrations of paroxetine. The cells viability, BrdU labeling and detection and the level of phosphorylatged cxtraeellular single-regulated kinase （pERKl/2） were measured by using the kit of CCK-8, immunocytochemieal method and western blot, respectively. Results The cell viability, number of BrdU＋ cells and expression of pERK1/2 in 10 IxmoL/L paroxetine treated groups were significantly increased compared to the control and other groups 48 h after treatment. However, there were no significant difterences in the cell viability, number of BrdU＋ cells and expression of pERK1/2 among paroxOine 5 μmoL/L, 21μmoL/L treated groups and control. Moreover, the cell viability was significantly decreased in 50 μmoL/L group. Conclusions Paroxetine can enhance the proliferation of the hippocampus-derived astroeytes via the ERK pathway.