中文摘要：

目的观察体外Transwell共培养系统中树突状细胞（dendritic cells,DCs）对神经干/前体细胞（neural stem/progenitor cells,NSPCs）增殖的影响,初步探讨神经营养因子-3（neurotrophin-3,NT-3）在DCs调控NSPCs中的作用机制。方法根据是否采用Transwell小室将DCs和NSPCs共培养分为分隔培养（DCs/NSPCs组）和混合培养（DCs＋NSPCs组）,ELISA法测定DCs组、NSPCs组、DCs＋NSPCs组、DCs/NSPCs组共培养48 h上清中的NT-3的含量。为观察NT-3在DCs调控NSPCs中的作用及可能的机制,成球法检测NSPCs组、NSPCs/DCs＋K252a组、NSPCs＋DCs组、NSPCs＋NT-3组的NSPCs增殖,免疫细胞荧光法和Western blot法检测各组NSPCs表面酪氨酸激酶受体C（TrkC）的表达。结果 ELISA实验结果显示,Transwell共培养48 h上清NT-3的含量,NSPCs/DCs组较DCs组和NSPCs组明显升高（P〈0.05）。镜下观察、测量结果显示NSPCs/DCs组和NSPCs＋NT-3组神经球数量增多,平均直径明显增大（P〈0.05）。免疫细胞荧光和Western blot检测结果表明,NSPCs＋DCs组和NSPCs＋NT-3组中NSPCs的TrkC表达较NSPCs组和NSPCs/DCs＋K252a组高（P〈0.05）。结论体外DCs与NSPCs共培养,促进NSPCs增殖、NT-3的分泌及TrkC的表达,NT-3可能是通过TrkC受体参与NSPCs增殖的调控。

英文摘要：

Objective To investigate the effect of dendritic cells（DCs） coculture on the proliferation of neural stem/progenitor cells（NSPCs） in vitro,and explore the role of TrkC receptor and neurotrophin-3（NT-3） in the process.Methods According to whether to adopt the Transwell chamber,the DCs and the NSPCs were co-cultured and divided into separate culture（DCs/NSPCs group） and mixed culture（DCs＋NSPCs group,without the use of Transwell chamber）.ELISA was used to detect neurotrophin-3（NT-3） protein in the supernatant in 48 h after culture in NSPCs＋DCs group（mixed culture）,DCs group,NSPCs group,and NSPCs/DCs group（only cells separated by Transwell chamber）.Tyrosine kinase receptors C（TrkC） proteins on the surface of NSPCs were detected by immunofluorescence staining and Western blotting analysis in NSPCs group,NSPCs/DCs＋K252a group（TrKC blocker）,NSPCs＋DCs group,and NSPCs＋NT-3 group.Results The levels of NT-3 in the supernatant were significantly higher in 48 h after culture in NSPCs/DCs group than in DCs group and NSPCs group（P0.05）.And microscopy showed significantly increases in the number and the average diameter of neurospheres in NSPCs/DCs group（P0.05）.Immunofluorescence staining and Western blotting indicated that the expression level of TrkC were significantly higher in NSPCs/DCs group and NSPCs＋DCs group than in NSPCs group and NSPCs/DCs＋K252a group（P0.05）.Conclusion DCs in coculture promotes NSPCs proliferation in vitro,which might be through TrkC-NT-3 signal pathway.