中文摘要：

在构建转基因红鲤的基础上,采用生物预警系统比较和分析了非转基因和转基因红鲤（Cyprinus carpio）在不同铜离子浓度条件下的游速和活动范围的变化。转基因红鲤和非转基因红鲤孵育自同一批受精卵,转基因红鲤体长（5.31±0.64）cm、体重（3.40±0.55）g,非转基因红鲤体长（4.58±0.59）cm、体重（2.40±0.58）g。CuSO4的浓度设置0（对照）、5、15、25、35和45μg/L共6组。生物预警系统包括储水池、数字摄像仪和数据运算处理器,可以记录鱼的二维移动轨迹,并计算出鱼的平均游速。试验结果表明,非转基因和转基因红鲤的平均游速分别为：对照组1、1.57 BL/s,5μg/L组1.24、1.07 BL/s,15μg/L组1.61、1.03 BL/s,25μg/L组1.50、1.59 BL/s,35μg/L组1.62、1.61 BL/s,45μg/L组1.25、1.97BL/s。非转基因和转基因红鲤的活动范围：除35μg/L组外,其他组的坐标X值非转基因与转基因红鲤间差异都极显著（P〈0.01）;对照组、5和25μg/L浓度组,转基因红鲤坐标X值极显著高于非转基因红鲤;15和45μg/L浓度组,非转基因红鲤坐标X值极显著高于转基因红鲤。低于45μg/L时,铜离子没有对转基因和非转基因红鲤产生明显的毒性;在45μg/L浓度组,转基因红鲤对铜离子不敏感,而非转基因红鲤较敏感。

英文摘要：

Copper is a major contaminant in water, and can affect the behavior and metabolism of fish. Cyprinus carpio is a common ornamental fish, and capable of adapting to poor quality water. To date, the effect of copper （II） on the behavior of Cyprinus carpio has not been reported. After establishing transgenic Cyprinus carpio, we compared the swimming behavior of both non-transgenic and transgenic Cyprinus carpio under different concentra- tions of copper （II） ions. The average swimming velocity （ASV） and the range of activity of non-transgenic and transgenic Cyprinus carpio of a control group （A） and five test groups （B-F） with CuSO4 concentrations of 5, 15, 25, 35 and 45 μg/L were compared. The initial body length and body weight of the transgenic test fish were （5.31±0.64） cm and （3.40 ±0.55 ） g and, for the non-transgenic fish, the values were （4..58± 0.59） cm and （2.40 ± 0.58 ） g. The experiment was carried out using a biological early warning system consisting of an aquarium e- quipped with a digital camera and a computer program was used to analyze the recorded motions of test fish. Analy- sis of fish movement gave ASV and two dimensional trajectories, with the X axis （CX） representing horizontal mo- tion and the Y axis （CY） representing vertical motion. The ASV for non-transgenic and transgenic Cyprinus carpio was, respectively, 1.00 and 1.57 BL/s （group A）, 1.24 and 1.07 BL/s （group B）, 1.61 and 1.03 BL/s （group C）, 1.50 and 1.59 BL/s （group D）, 1.62 and 1.61 BL/s （group E）, 1.25 and 1.97 BL/s （group F）. The average swimming velocity of transgenic Cyprinus carpio was significantly higher than that of non-transgenic Cyprinus carpio in the control groups, consistent with the strong skeletal muscles of transgenic fish. However, at CuSO4 treatment levels of 5 μg/L and 15 μg/L, the swimming velocity of non-transgenic fish was significantly high- er than that of transgenic fish and its control group （ P 〈 0.01 ） , indicating that the