中文摘要：

采用荧光实时定量PCR技术研究饥饿及复投喂对大黄鱼不同组织中两型肌肉生长抑制素（Myostatin,MSTN）基因表达的影响。结果显示,肌肉中MSTN-1和MSTN-2在饥饿0—35d期间,表达量先升高后降低,分别在21d和28d达到最高,复投喂时期逐渐下降;脑中两型MSTN表达变化不同,但都在饥饿35d表达量最低,复投喂时期表达显著上升,与对照组有显著差异;脾脏中MSTN-1在饥饿28d表达量最高,MSTN-2在饥饿21d表达量最高,复投喂时期表达都逐渐降低;肾脏中两型MSTN表达变化相似,都在饥饿21d降到最低,然后升高;肠中1型和2型分别在饥饿14d和7d表达量最低,在28d表达量都达到最高;肝脏中两型MSTN都是在饥饿21d表达量最低,之后显著升高,复投喂时期表达上升。结果提示两型MSTN在不同组织中的功能可能存在差异。

英文摘要：

In this study, we used real-time PCR to investigate the effects of fasting and re-feeding on the expression of two MSTN genes in large yellow croaker（Larimichthys crocea）. The results showed that during the 35-day fasting treatment, the expression of MSTN-1 and MSTN-2 in skeletal muscles firstly increased and then decreased, and their expressions perked on the 21 st and the 28 th day respectively. The expressions were down-regulated during the re-feeding period. The expression patterns of the two MSTNs were different in the brain, although they both reached the lowest level on the 35 th day and then increased after re-feeding. In the spleen the expressions of MSTN-1 and MSTN-2 reached to the highest levels on the 28 th and the 21 st days respectively, and both declined after the re-feeding. In kidney the expression patterns of the two genes were similar, and they both reached the lowest on the 21 st day after fasting followed by an increase. The expression of MSTN-1 and MSTN-2 in the intestine decreased significantly on the 14 th and the 7th day respectively, and then peaked on the 28 th day. In the liver, the expression of MSTN-1 and MSTN-2 decreased sharply on the 21 st day and then increased. The expressions of both genes were up-regulated after re-feeding. Our findings suggested that the functions of the two MSTN genes might be various in different tissues.