中文摘要：

目的：探讨去甲斑蝥素对环氧合酶-2（COX-2）过表达人肠癌移植瘤裸鼠模型的抑制作用。方法：建立COX-2过表达的人肠癌移植瘤裸鼠模型,观察去甲斑蝥素（NCTD）对瘤体生长、裸鼠生存期和COX-2表达的影响。结果：成功建立COX-2过表达的移植瘤裸鼠模型,且HCT-116/COX-2实验组中COX-2蛋白表达显著上调,与对照组比较具有统计学差异（P〈0.05）;HCT-116/COX-2组的瘤体生长速度明显高于HCT-116组及空质粒组,差异有统计学意义（P〈0.05）;COX-2过表达组生存天数明显少于HCT-116组和空质粒组,差异有统计学差异（P〈0.05）;NCTD对COX-2过表达裸鼠瘤体生长有明显的抑制作用,并且呈剂量依赖性;NCTD低剂量组、中剂量组、高剂量组裸鼠平均生存天数分别为（80.6±6.9）、（92.8±16.5）、（93.4±14.5）d,与HCT-116-COX-2（75.7±11.2）d比较均有统计学差异（P〈0.05）;NCTD对COX-2的表达有明显抑制作用,且呈剂量依赖性。结论：COX-2对人结肠癌裸鼠移植瘤的生长有明显的促进作用,且降低了裸鼠的生存期;NCTD能够抑制裸鼠移植瘤的生长,呈现剂量依赖性,并能延长荷瘤裸鼠的生存期,可能通过降低COX-2的表达抑制裸鼠结肠癌的生长。

英文摘要：

Objective： To investigate the effects of norcantharidin on xenografts tumor nude mice of human colorectal cancer with COX-2 over-expression,for providing experimental evidence of traditional Chinese medicine on prevention and treatment in colorectal cancer.Methods： Establishing xenografts tumor nude mice model of human colorectal cancer with COX-2 over-expression,to observe the effect of norcantharidin on tumor growth,survival time of nude mice and COX-2 expression.Results： Successfully established xenografts tumor nude mice model of human colorectal cancer with COX-2 over-expression.Compared with the model group,COX-2 protein expression in HCT-116/COX-2 group was significantly up-regulated,the difference was statistically significance（P0.05）;tumor growth rate in HCT-116/COX-2 group was significantly higher than the HCT-116 group and empty plasmid group,the difference was statistically significance（P0.05）;survival time of COX-2 over-expression group was significantly less than the HCT-116 group and the empty plasmid group with statistical significance（P0.05）;Norcantharidin significantly inhibited the tumor growth in nude mice with COX-2 over-expression in a dose-dependent manner;the average survival days of nude mice with COX-2 over-expression was 75.7±11.2,but the norcantharidin groups with different doses were（80.6±6.9） days,（92.8±16.5） days,（93.4±14.5） days from the low dose group to the middle dose group and high dose groups,which were all statistically significant compared with HCT-116/COX-2 group（P0.05）;norcantharidin could significantly inhibited the COX-2 expression in a dose-dependent manner.Conclusion： COX-2 significantly promoted the growth of xenografts tumor in nude mice,and reduced the survival time of nude mice;norcantharidin could inhibit the growth of xenografts tumor in nude mice in a dose-dependent manner,and extend the survival time of nude mice.Norcantharidin might inhibit tumor growth by inhibiting the expression of COX-2.