中文摘要：

目的：探讨CD4＋CD29T细胞在湿热型溃疡性结肠炎中的表达及意义。方法：按照年龄及性别构成无统计学差异的原则，将临床观察对象入选分为正常组（n=32）、单纯湿热组（n=18）、非湿热型UC组（n=29）及湿热型UC组（n=16）共4组；将40只SD大鼠随机分为健康组（n=10）、单纯湿热组（n=10）、非湿热型UC组（n=10）及湿热型UC组（n=10）共4组，采用高脂、高糖饲料联合人工气候箱法造出大鼠湿热型模型，再结合三硝基苯磺酸（TNBS）灌肠法制作湿热型UC模型，非湿热型UC组单独用TNBS法。使用流式细胞分析仪检测以上各组患者和大鼠外周血CD4＋CD29T细胞所占的百分比。结果：单纯湿热组、非湿热型UC组和湿热型UC组患者和大鼠外周血中CD4＋CD29T细胞的表达均显著升高（P〈0．05），其中以湿热型UC组表达最高。结论：CD4＋CD29T细胞在湿热型UC患者和大鼠中表达显著升高，可能参与了湿热型UC的发病。

英文摘要：

Objective：The objective of this study is to investigate expressions of CD4＋CD29Teells in ulcerative colitis （UC） with damp - heat syndrome patients and rats. Methods ： We studied 18 patients with damp - heat syndrome ,29 UC patients, 16 UC patients with damp - heat syndrome and 32 healthy controls. 40 SD rats were distributed into four groups, containing control group （ n = 10 ） , damp - heat syndrome group （ n = 10, induced by giving the high sugar fat food and artificial bioclimatic chamber）, TN- BS group （ n = lO,induced by TNBS） and TNBS ＋ damp - heat syndrome group （ n = 10, induced by damp - heat syndrome meth- ode with TNBS）. The frequency of CD4 CD29T cells in peripheral blood mononuclear cells （PBMCs） was evaluated by flow cyto- metric analysis. Results：The results demonstrated a significant increase in the frequency of CD4＋ CD29T ceils in patients and rats with the damp - heat syndrome, UC or UC with damp - heat syndrome compared with healthy controls. Conclusion ： CD4＋ CD29T cells are involved in the pathogenesis of UC with damp- heat syndrome. Increased CD4 CD29T cells in the peripheral blood may be an important factor in the development of UC.